Studies on circulating met-enkephalin and beta-endorphin: normal subjects and patients with renal and adrenal disease
| Autor: | G. M. Besser, Roger Smith, Vicky Clement-Jones, J. Mallinson, Ashley B. Grossman, P. J. Lowry, R. C. Gaillard, Lesley H. Rees, Sally J. Ratter |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Met-enkephalin
Male beta-Lipotropin medicine.medical_specialty endocrine system Hydrocortisone Endocrinology Diabetes and Metabolism medicine.medical_treatment Enkephalin Methionine Adrenocorticotropic hormone Dexamethasone Cushing syndrome chemistry.chemical_compound Endocrinology Addison Disease Adrenocorticotropic Hormone Internal medicine medicine polycyclic compounds Humans Insulin Endorphins Cushing Syndrome Beta-Lipotropin Adrenalectomy digestive oral and skin physiology beta-Endorphin Enkephalins medicine.disease chemistry nervous system Kidney Failure Chronic hormones hormone substitutes and hormone antagonists medicine.drug |
| Popis: | Studies were performed to define the responses of plasma met-enkephalin to various endocrine and pathological stimuli and to determine the relationship 0,14656 between plasma β-endorphin and met-enkephalin. During insulin-induced hypoglycaemia ACTH, β-LPH and β-endorphin immunoreactivity rose in parallel, but plasma met-enkephalin did not change significantly. Sephadex G75 chromatography of samples taken at the time of the peak response (45 min) confirmed the rise in both β-LPH and β-endorphin. During administration of dexamethasone, 0.5 mg 6 hourly for 48 h, plasma cortisol and ACTH became undetectable at 24 h, and β-LPH and β-endorphin fell 0,15159 significantly by 24 h and were undetectable by 48 h; plasma met-enkephalin, however, showed no significant change. Nine adrenalectomized patients with Cushing's disease and four patients with Addison's disease had elevated plasma ACTH, β-LPH and β-endorphin but normal plasma met-enkephalin levels. Each of ten patients with renal failure had markedly elevated plasma met-enkephalin immunoreactivity which co-eluted with synthetic met-enkephalin on BioGel P4 chromatography. Trypsin and carboxypeptidase-B 0,15663 digestion of the P4 chromatography fractions generated met-enkephalin immunoreactivity in earlier fractions, indicating the presence of a potential high molecular weight met-enkephalin precursor. The results imply that different control mechanisms govern β-endorphin and met-enkephalin plasma levels and that the adrenal is not the only source of plasma met-enkephalin. The elevated levels of met-enkephalin in renal failure may be due to impaired clearance of met-enkephalin or of its precursor or to 0,16168 increased met-enkephalin production. |
| Databáze: | OpenAIRE |
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