Flavobacterium tistrianum sp. nov., a gliding bacterium isolated from soil
| Autor: | Sophea Pheng, Kanjana Rueangyotchanthana, Bundit Fungsin, Chantara Phoonsiri, Song-Gun Kim, Suksan Srichuay, Chatrudee Suwannachart |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine DNA Bacterial Gliding motility Sequence analysis Phosphatidylserines 010603 evolutionary biology 01 natural sciences Microbiology Flavobacterium 03 medical and health sciences stomatognathic system RNA Ribosomal 16S Ecology Evolution Behavior and Systematics Phylogeny Soil Microbiology Base Composition biology Strain (chemistry) Phosphatidylethanolamines Fatty Acids Nucleic Acid Hybridization Vitamin K 2 General Medicine Sequence Analysis DNA Ribosomal RNA 16S ribosomal RNA biology.organism_classification Thailand Bacterial Typing Techniques 030104 developmental biology Soil microbiology Bacteria |
| Zdroj: | International journal of systematic and evolutionary microbiology. 66(6) |
| ISSN: | 1466-5034 |
| Popis: | A novel gliding bacterial strain, GB 56.1T, was obtained from soil at the Sakaerat Biosphere Reserve, in Nakhon Ratchasima province, Thailand; the strain was characterized using a polyphasic approach. Cells were Gram-stain-negative, yellow, rod shaped and devoid of flagella, but showed gliding motility. Phylogenetic analysis based on 16S rRNA gene sequences found that GB 56.1T was a member of the genus Flavobacterium and that the strain shared the highest sequence similarities with Flavobacterium nitrogenifigens (98.4 %), Flavobacterium anhuiense(98.3 %) and Flavobacterium ginsenosidimutans (97.9 %). The similarities of the sequences of all other species of the genus Flavobacterium were below 97.4 %. The major respiratory quinone of strain GB 56.1T was MK-6; fatty acids were iso-C15:0, C16:1ω6c/C16:1ω7c, C16:0 and C16:0 3-OH. The major polar lipids were phosphatidylethanolamine, phosphatidylserine, an unidentified amino lipid and four polar lipids. The DNA G+C content of this strain was 34.2 mol%. The DNA-DNA relatedness of GB 56.1T was highest against F.anhuiense, with a value of 37.6 %. On the basis of morphological, physiological and chemotaxonomic characteristics, DNA-DNA hybridization relatedness and 16S rRNA gene sequence analysis, we conclude that strain GB 56.1T represents a novel species, for which the name Flavobacterium tistrianum sp. nov. is proposed. The type strain is GB 56.1T (=TISTR 1612T =KCTC 42679T). |
| Databáze: | OpenAIRE |
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