Evidence for a higher resolution of HLA genotyping by a new NGS-based approach
| Autor: | Alizadeh, M., Walencik, A., Frassati, C., Moskovtchenko, P., Lafarge, X., Verite, F., Semana, G, Alizadeh, G, Walencik, F, Frassati, F, Moskovtchenko, F, Lafarge, F, Verite, G |
|---|---|
| Přispěvatelé: | Eindhoven University of Technology [Eindhoven] (TU/e), Etablissement Français du Sang - Alpes-Méditerranée (EFS - Alpes-Méditerranée), Etablissement Français du Sang, Laboratoire d'hémathologie cellulaire - EFS BFC, Etablissement français du sang [Bourgogne-Franche-Comté] (EFS BFC), Centre de Transfusion Sanguine Aquitaine-Limousin (CTS AQUITAINE-LIMOUSIN), Centre de Transfusion Sanguine, Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Etablissement français du sang [Rennes] (EFS Bretagne), Commissariat à l'énergie atomique et aux énergies alternatives - Laboratoire d'Electronique et de Technologie de l'Information (CEA-LETI), Direction de Recherche Technologique (CEA) (DRT (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Centre d'Etude de Saclay, Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Etablissement français du sang [Bourgogne-Franche-Comté] (EFS [Bourgogne-Franche-Comté]) |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Human leucocyte antigen Genotyping Techniques Sequence analysis [SDV]Life Sciences [q-bio] Genes MHC Class II Clinical Biochemistry Genes MHC Class I Human leukocyte antigen Biology Génotypage de haute résolution Polymerase Chain Reaction 03 medical and health sciences 0302 clinical medicine HLA Antigens Humans Allele Alleles Genetics Transplantation Histocompatibility Testing Biochemistry (medical) High-Throughput Nucleotide Sequencing High-resolution genotyping Hematology HLA 030104 developmental biology NGS Hla genotyping [SDV.IMM]Life Sciences [q-bio]/Immunology Human genome Software 030215 immunology |
| Zdroj: | Transfusion Clinique et Biologique Transfusion Clinique et Biologique, 2017, 24 (3), pp.120-123. ⟨10.1016/j.tracli.2017.05.011⟩ Transfusion clinique et biologique : journal de la Societe francaise de transfusion sanguine Transfusion clinique et biologique : journal de la Societe francaise de transfusion sanguine, 2017, 24 (3), pp.120-123. ⟨10.1016/j.tracli.2017.05.011⟩ |
| ISSN: | 1246-7820 1953-8022 |
| DOI: | 10.1016/j.tracli.2017.05.011 |
| Popis: | International audience; With more than 16,000 alleles identified, the human leucocyte antigen (HLA) system is one of the most polymorphic regions of the human genome. Regarding the crucial role of HLA compatibility in transplantation and especially in Hematopoietic Stem Cell Transplantation, identification of HLA polymorphisms at a high-resolution level is of major interest. Recently, NGS technology has been proposed which appears to be simpler and more informative than the classical molecular methods such as SSP, SSOr and SBT. In the present report, a new set of NGS reagents and the appropriate associated software for sequence analysis are described. Through different studies, the performances of the system are illustrated and demonstrate that the method herein described overcomes current limitations in performing high-resolution HLA typing in clinical laboratories. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect