Transcriptomic analysis of microRNAs–mRNAs regulating innate immune response of zebrafish larvae against Vibrio parahaemolyticus infection
| Autor: | Weiming Li, Ce Ji, Jianfeng Ren, Qinghua Zhang, Xinya Guo, Yao Zu |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Heme binding Aquatic Science Axonogenesis Fish Diseases 03 medical and health sciences Animals Environmental Chemistry RNA Messenger KEGG Organophosphate metabolic process Zebrafish Innate immune system biology Tetrapyrrole binding Gene Expression Profiling 04 agricultural and veterinary sciences General Medicine biology.organism_classification Immunity Innate Cell biology MicroRNAs 030104 developmental biology Protein dimerization activity Vibrio Infections 040102 fisheries 0401 agriculture forestry and fisheries Vibrio parahaemolyticus |
| Zdroj: | Fish & Shellfish Immunology. 91:333-342 |
| ISSN: | 1050-4648 |
| DOI: | 10.1016/j.fsi.2019.05.050 |
| Popis: | In recent years, microRNAs (miRNAs) have been shown to play important roles in immunity. Analyses of the functions of miRNAs and their targets are useful in understanding the regulation of the immune response. To understand the relationships between miRNAs and their targets during infection, we used zebrafish as an infection model in which to characterize the miRNA and mRNA transcriptomes of zebrafish larvae infected with Vibrio parahaemolyticus. We identified the differentially expressed miRNAs and mRNAs. Overall, 37 known zebrafish miRNAs were differentially expressed in the infection group and 107 predicted target genes of 26 miRNAs were differentially expressed in the mRNA transcriptome. These targets with specific Gene Ontology (GO) terms, such as peripheral nervous system neuron axonogenesis, organophosphate metabolic process, heme binding, protein binding, tetrapyrrole binding, protein dimerization activity, and aromatase activity, which regulate nerve conduction, energy metabolism, hematopoiesis, and protein synthesis. They were also associated with Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways such as phototransduction, tryptophan metabolism, notch signaling, and purine metabolism. Our findings indicate that miRNAs regulate the innate immune response via complex networks, and zebrafish (Danio rerio, dre)-miR-205-3p, dre-miR-141-5p, dre-miR-200a-5p, dre-miR-92a-2-5p, dre-miR-192, and dre-miR-1788 may play important roles in the innate immune response by regulating target genes. |
| Databáze: | OpenAIRE |
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