Regulation of endotoxin-induced inhibition of macrophage migration by fresh serum
| Autor: | D H Heilman |
|---|---|
| Rok vydání: | 1977 |
| Předmět: |
Lipopolysaccharides
Hot Temperature Guinea Pigs Immunology Enterotoxin Absorption (skin) Biology Microbiology Guinea pig chemistry.chemical_compound Escherichia coli Animals Ascitic Fluid Macrophage Cells Cultured Macrophages Pokeweed mitogen Polysaccharides Bacterial Zymosan Complement System Proteins Salmonella typhi Molecular biology Endotoxins Infectious Diseases chemistry Concanavalin A Cell Migration Inhibition biology.protein Parasitology Mitogens Antibody Research Article |
| Zdroj: | Infection and Immunity. 17:371-377 |
| ISSN: | 1098-5522 0019-9567 |
| DOI: | 10.1128/iai.17.2.371-377.1977 |
| Popis: | Purified endotoxin (LPS) caused macrophage migration inhibition (MMI) in capillary tube cultures of guinea pig peritoneal macrophages in medium prepared with 15% fresh-frozen guinea pig serum. The inactivation of serum by heating at 56 degrees C for 30 min or by zymosan absorption prevented LPS-induced MMI. LPS was fully inhibitory in fresh C4-deficient guinea pig serum. Heat treatment of normal serum at 50 to 52 degrees C for 30 min to inactivate the alternate complement (C) pathway prevented or significantly decreased LPS-induced MMI, but heating C4-deficient serum at 50 to 52 degrees C for 30 min prevented LPS-MMI in all instances. These results suggest that the reaction was effected via the alternate C pathway but that some inhibition of migration was permitted via the classical C pathway, presumably due to antibodies for LPS in some normal sera. Pretreatment of normal serum with cobra venom factor decreased or prevented LPS-MMI in most instances, but similar results were obtained with C4-deficient serum. Experiments with chelated sera were unsuccessful because of the immobilization of macrophages by 10 mM ethylenediamine-tetraacetic acid and by 10 mM Mg-ethyleneglycol-bis (beta-aminoethyl)-N,N-tetraacetic acid. Low doses of concanavalin A and staphylococcal enterotoxin B and large doses of pokeweed mitogen caused MMI in "inactivated serum" medium, but MMI was enhanced in fresh serum. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|