Transcriptome Analysis of Alkali Shock and Alkali Adaptation inListeria monocytogenes10403S
| Autor: | Senthil Natesan, Ian S. Blair, David A. McDowell, Brian J. Wilkinson, Efstathios S. Giotis, Arunachalam Muthaiyan |
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| Rok vydání: | 2010 |
| Předmět: |
Adaptation
Biological Biology medicine.disease_cause Polymerase Chain Reaction Applied Microbiology and Biotechnology Microbiology Transcriptome Listeria monocytogenes Transcription (biology) medicine RNA Messenger Gene Pathogen Oligonucleotide Array Sequence Analysis Messenger RNA Gene Expression Profiling Original Articles Gene Expression Regulation Bacterial Hydrogen-Ion Concentration biology.organism_classification Gene expression profiling RNA Bacterial Listeria Animal Science and Zoology Food Science |
| Zdroj: | Foodborne Pathogens and Disease. 7:1147-1157 |
| ISSN: | 1556-7125 1535-3141 |
| DOI: | 10.1089/fpd.2009.0501 |
| Popis: | Alkali stress is an important means of inactivating undesirable pathogens in a wide range of situations. Unfortunately, Listeria monocytogenes can launch an alkaline tolerance response, significantly increasing persistence of the pathogen in such environments. This study compared transcriptome patterns of alkali and non-alkali-stressed L. monocytogenes 10403S cells, to elucidate the mechanisms by which Listeria adapts and/or grows during short- or long-term alkali stress. Transcription profiles associated with alkali shock (AS) were obtained by DNA microarray analysis of midexponential cells suspended in pH 9 media for 15, 30, or 60 min. Transcription profiles associated with alkali adaptation (AA) were obtained similarly from cells grown to midexponential phase at pH 9. Comparison of AS and AA transcription profiles with control cell profiles identified a high number of differentially regulated open-reading frames in all tested conditions. Rapid (15 min) changes in expression included upregulation of genes encoding for multiple metabolic pathways (including those associated with Na+/H+ antiporters), ATP-binding cassette transporters of functional compatible solutes, motility, and virulence-associated genes as well as the σ(B) controlled stress resistance network. Slower (30 min and more) responses to AS and adaptation during growth in alkaline conditions (AA) involved a different pattern of changes in mRNA concentrations, and genes involved in proton export. |
| Databáze: | OpenAIRE |
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