The synergistic mechanism of total saponins and flavonoids in Notoginseng−Safflower pair against myocardial ischemia uncovered by an integrated metabolomics strategy
| Autor: | Zhiyong Du, Peng Gao, Yan Li, Jin-Yang Song, Yu-Qing Meng, Xiao-Yu Guo, Peng-Fei Tu, Ying-Yuan Lu, Yong Jiang |
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| Rok vydání: | 2020 |
| Předmět: |
Male
0301 basic medicine Myocardial ischemia Antioxidant medicine.medical_treatment Carthamus tinctorius Panax notoginseng RM1-950 Pharmacology Notoginseng total saponins medicine.disease_cause Synergistic effects Synergistic mechanism Proinflammatory cytokine Rats Sprague-Dawley 03 medical and health sciences 0302 clinical medicine Metabolomics medicine Animals Flavonoids biology Chemistry Myocardium Carthamus Safflower total flavonoids Drug Synergism Heart General Medicine Saponins biology.organism_classification 030104 developmental biology 030220 oncology & carcinogenesis Therapeutics. Pharmacology Oxidative stress |
| Zdroj: | Biomedicine & Pharmacotherapy, Vol 130, Iss, Pp 110574-(2020) |
| ISSN: | 0753-3322 |
| Popis: | The Notoginseng-Safflower pair composed of Panax notoginseng (Burk.) F. H. Chen and Carthamus tinctorius L. has remarkable clinical efficacy for preventing and treating cardiovascular diseases in China. Notoginseng total saponins (NS) and Safflower total flavonoids (SF) are the major effective ingredients in Notoginseng and Safflower, respectively. Though our previous study showed that the combination of NS and SF (NS-SF) exhibits significant cardioprotective effects for myocardial ischemia (MI), there might be difference in their action mechanisms. However, the anti-MI characteristics of individual NS and SF remains unclear. Herein, an integrated metabolomics strategy coupled with multiple biological methods were employed to investigate the cardioprotective effects of NS and SF alone or in combination against isoproterenol (ISO)-induced MI and to further explore the synergistic relationship between NS and SF. Our results demonstrated that pretreatments with NS, SF, and NS-SF all showed cardioprotective effects against MI injury and NS-SF exhibited to be the best. Interestingly, the results demonstrated that NS and SF exhibited differentiated metabolic targets and mediators in the glycerophospholipid metabolism. Furthermore, administration of NS alone exhibited greater effects on reversing the elevated the proinflammatory metabolites and mediators in MI rats compared to SF alone. However, individual SF showed greater amelioration of MI-disturbed antioxidant and prooxidative metabolites and better inhibition of the oxidative stress than NS alone. Collectively, our study demonstrated that the capability of NS-SF to regulate both metabolic targets of NS and SF might be the basis of NS-SF to produce a cooperative effect greater than their individual effects that enhance the anti-MI efficacy and provided valuable information for the clinical application of Notoginseng-Safflower pair. |
| Databáze: | OpenAIRE |
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