Novel chitin scaffolds derived from marine sponge Ianthella basta for tissue engineering approaches based on human mesenchymal stromal cells: Biocompatibility and cryopreservation
| Autor: | Alexander G. Tonevitsky, Hermann Ehrlich, Sergey Nikulin, Vitalii Mutsenko, Peter J. Schupp, Stephan Meschke, Vasilii V. Bazhenov, Sophie Brüggemeier, Heike Meißner, Anja Lode, Lothar Lauterboeck, Dmitriy N. Tarusin, Kathleen Schütz, Elke Gossla, Alexander Y. Petrenko, Sergey Rodin, Allison L. Stelling, Birgit Glasmacher, Ashwini Rahul Akkineni, Olena Rogulska, Oleksandr Gryshkov, Jane Fromont, Michael Gelinsky, Konstantin R. Tabachnik |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Biocompatibility Biocompatible Materials Chitin 02 engineering and technology Biochemistry 03 medical and health sciences chemistry.chemical_compound Tissue engineering Ianthella basta Structural Biology Materials Testing Spectroscopy Fourier Transform Infrared Animals Humans Molecular Biology Cryopreservation Adipogenesis Tissue Engineering Tissue Scaffolds biology Chemistry Mesenchymal stem cell Biomaterial Cell Differentiation Mesenchymal Stem Cells General Medicine Anatomy 021001 nanoscience & nanotechnology biology.organism_classification Porifera Cell biology Sponge 030104 developmental biology Stem cell 0210 nano-technology |
| Zdroj: | International Journal of Biological Macromolecules. 104:1955-1965 |
| ISSN: | 0141-8130 |
| DOI: | 10.1016/j.ijbiomac.2017.03.161 |
| Popis: | The extraordinary biocompatibility and mechanical properties of chitinous scaffolds from marine sponges endows these structures with unique properties that render them ideal for diverse biomedical applications. In the present work, a technological route to produce "ready-to-use" tissue-engineered products based on poriferan chitin is comprehensively investigated for the first time. Three key stages included isolation of scaffolds from the marine demosponge Ianthella basta, confirmation of their biocompatibility with human mesenchymal stromal cells, and cryopreservation of the tissue-like structures grown within these scaffolds using a slow cooling protocol. Biocompatibility of the macroporous, flat chitin scaffolds has been confirmed by cell attachment, high cell viability and the ability to differentiate into the adipogenic lineage. The viability of cells cryopreserved on chitin scaffolds was reduced by about 30% as compared to cells cryopreserved in suspension. However, the surviving cells were able to retain their differentiation potential; and this is demonstrated for the adipogenic lineage. The results suggest that chitin from the marine demosponge I. basta is a promising, highly biocompatible biomaterial for stem cell-based tissue-engineering applications. |
| Databáze: | OpenAIRE |
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