Lower sperm DNA fragmentation after r-FSH administration in functional hypogonadotropic hypogonadism
| Autor: | Ettore Cittadini, Maria Carmela Roccheri, Liana Bosco, Anna Maria Brucculeri, Salvatore Longobardi, Giovanni Ruvolo |
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| Přispěvatelé: | Ruvolo, G, Roccheri, MC, Brucculeri, AM, Longobardi, S, Cittadini, E, Bosco, L |
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Adult
Male endocrine system medicine.medical_specialty DNA Fragmentation Asthenozoospermia law.invention Andrology Hypogonadotropic hypogonadism law Gamete Biology Internal medicine Genetics medicine Humans Apoptosis Gametogenesis DNA Sperm Settore BIO/06 - Anatomia Comparata E Citologia Genetics (clinical) Gametogenesis Sperm Count business.industry Hypogonadism Obstetrics and Gynecology Oligospermia General Medicine medicine.disease Spermatozoa Sperm Recombinant Proteins Endocrinology Reproductive Medicine Apoptosis Recombinant DNA DNA fragmentation Follicle Stimulating Hormone Human business Developmental Biology |
| Popis: | PURPOSE: An observational clinical and molecular study was designed to evaluate the effects of the administration of recombinant human FSH on sperm DNA fragmentation in men with a non-classical form of hypogonadotropic hypogonadism and idiopathic oligoasthenoteratozoospermia. METHODS: In the study were included 53 men with a non-classical form of hypogonadotropic hypogonadism and idiopathic oligoasthenoteratozoospermia. In all patients, sperm DNA fragmentation index (DFI), assessed by terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate (dUTP) in situ DNA nick end-labelling (TUNEL) assay, was evaluated before starting the treatment with 150 IU of recombinant human FSH, given three times a week for at least 3 months. Patients' semen analysis and DNA fragmentation index were re-evaluated after the 3-month treatment period. RESULTS: After recombinant human FSH therapy, we did not find any differences in terms of sperm count, motility and morphology. The average DNA fragmentation index was significantly reduced (21.15 vs 15.2, p 15 %), while no significant variation occurred in the patients with DFI values ≤15 %. CONCLUSIONS: Recombinant human FSH administration improves sperm DNA integrity in hypogonadotropic hypogonadism and idiopathic oligoasthenoteratozoospermia men with DNA fragmentation index value >15 % . |
| Databáze: | OpenAIRE |
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