Author Correction: 58-F, a flavanone from Ophiopogon japonicus, prevents hepatocyte death by decreasing lysosomal membrane permeability
| Autor: | Xu-Dong Hu, Xiao-Feng Yan, Xiao-Ling Wang, Ting-Jie Ye, Pei Zhao |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Lysosomal membrane
Science Ophiopogon japonicus Permeability chemistry.chemical_compound Mice medicine Animals Author Correction Mice Inbred BALB C Multidisciplinary biology Cell Death Carbon Tetrachloride Poisoning Ophiopogon Hydrogen Peroxide Intracellular Membranes biology.organism_classification Flavones Molecular biology medicine.anatomical_structure chemistry Permeability (electromagnetism) Hepatocyte Hepatocytes Medicine Lysosomes Flavanone |
| Zdroj: | Scientific Reports, Vol 11, Iss 1, Pp 1-3 (2021) Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | Lysosome membrane permeabilization (LMP) has been implicated in cell death. In the present study, we investigated the relationship between cell death and H2O2-/CCl4-induced LMP in hepatocytes in vitro and following acute liver injury in vivo. The key finding was that H2O2 triggered LMP by oxidative stress, as evidenced by a suppression of LAMP1 expression, a reduction in LysoTracker Green and AO staining, and the leakage of proton and cathepsin B/D from the lysosome to the cytoplasm, resulting in cell death. CCl4 also triggered hepatocyte death by decreasing lysosome LAMP1 expression and by inducing the accumulation of products of peroxidative lipids and oxidized proteins. Furthermore, a novel compound 5,8-dimethoxy-6-methyl-7-hydroxy-3-3(2-hydroxy-4-methoxybenzyl) chroman-4-one (58-F) was extracted from Ophiopogon japonicus and served as a potential therapeutic drug. In vivo and in vitro results showed that 58-F effectively rescued hepatocytes by decreasing LMP and by inducing lysosomal enzyme translocation to the cytosol. |
| Databáze: | OpenAIRE |
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