Species- and Strain-Specific Adaptation of the HSP70 Super Family in Pathogenic Trypanosomatids
| Autor: | Gerald F. Späth, Pierre Lechat, Jean-Claude Dujardin, Najma Rachidi, Tomáš Skalický, Julius Lukeš, Hideo Imamura, Alexis Criscuolo, Sima Drini |
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| Přispěvatelé: | Parasitologie moléculaire et Signalisation / Molecular Parasitology and Signaling, Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Hub Bioinformatique et Biostatistique - Bioinformatics and Biostatistics HUB, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Department of Biomedical Sciences [Antwerp], Institute of Tropical Medicine [Antwerp] (ITM), Institute of Parasitology, Czech Academy of Sciences [Prague] (CAS), University of South Bohemia, Institute of Parasitology [České Budějovice] (BIOLOGY CENTRE CAS), Biology Centre of the Czech Academy of Sciences (BIOLOGY CENTRE CAS), Czech Academy of Sciences [Prague] (CAS)-Czech Academy of Sciences [Prague] (CAS), Canadian Institute for Advanced Research (CIFAR), This work was supported by the French Government’s Investissements d’Avenir programme: Laboratoire d’Excellence ‘Integrative Biology of Emerging Infectious Diseases’ (Grant no. ANR-10-LABX-62-IBEID to G.F.S.), KALADRUG-R (EU/FP7-222895 to J.C.D.), the Belgian Development Cooperation (FA3 II VL control and FA3 project 95502 to J.C.D.), the Belgian Science Policy Office (TRIT, P7/41 to J.C.D.), the Flemish Fund for Scientific Research (G.0.B81.12 to J.C.D.), the INBEV-Baillet Latour foundation, and EWI (GEMINI and SINGLE grants to ITM SOFI-B to J.C.D.), the Czech Grant Agency (Grant 14-23986S to J.L.), a fellowship from the Pasteur–Paris University (PPU) International PhD program and the Institut Carnot Pasteur Maladies Infectieuses (to S.D.), and a Bourse Fin de thèse scientifique from the Fondation de Recherche Médicale (contract FDT20150532765 to S.D.), The authors thank Sophie Veillault for editorial help, Simonetta Gribaldo for discussion, and Mariette Matondo from the Institut Pasteur Proteomics platform for proteomics data analysis., ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010), Lechat, Pierre, Integrative Biology of Emerging Infectious Diseases - - IBEID2010 - ANR-10-LABX-0062 - LABX - VALID, Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Parasitologie moléculaire et Signalisation, University of Antwerp (UA), Clinical sciences, Medical Genetics, Vriendenkring VUB |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
MESH: Leishmaniasis heat shock protein phylogeny Genome MESH: Gene Amplification Gene duplication MESH: Animals Copy-number variation MESH: Leishmania / genetics MESH: Phylogeny MESH: HSP70 Heat-Shock Proteins Leishmaniasis MESH: Evolution Molecular HSP70 Genetics Leishmania MESH: Leishmaniasis / genetics Phylogenetic tree [SDV.BID.EVO]Life Sciences [q-bio]/Biodiversity/Populations and Evolution [q-bio.PE] synteny copy number variation [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology MESH: DNA Copy Number Variations Research Article MESH: Gene Amplification / genetics Protein family DNA Copy Number Variations MESH: Leishmania Locus (genetics) Biology MESH: Leishmaniasis / parasitology MESH: HSP70 Heat-Shock Proteins / genetics Evolution Molecular 03 medical and health sciences Species Specificity Phylogenetics parasitic diseases evolution [SDV.BID.EVO] Life Sciences [q-bio]/Biodiversity/Populations and Evolution [q-bio.PE] Animals Humans MESH: Species Specificity HSP70 Heat-Shock Proteins MESH: Genome [SDV.MP] Life Sciences [q-bio]/Microbiology and Parasitology Ecology Evolution Behavior and Systematics Synteny MESH: Humans 030102 biochemistry & molecular biology Gene Amplification gene loss 030104 developmental biology Human medicine MESH: DNA Copy Number Variations / genetics MESH: Leishmania / pathogenicity |
| Zdroj: | Genome Biology and Evolution Genome Biology and Evolution, Society for Molecular Biology and Evolution, 2016, 8 (6), pp.1980-1995. ⟨10.1093/gbe/evw140⟩ Genome Biology and Evolution, 2016, 8 (6), pp.1980-1995. ⟨10.1093/gbe/evw140⟩ |
| ISSN: | 1759-6653 |
| Popis: | International audience; All eukaryotic genomes encode multiple members of the heat shock protein 70 (HSP70) family, which evolved distinctive structural and functional features in response to specific environmental constraints. Phylogenetic analysis of this protein family thus can inform on genetic and molecular mechanisms that drive species-specific environmental adaptation. Here we use the eukaryotic pathogen Leishmania spp. as a model system to investigate the evolution of the HSP70 protein family in an early-branching eukaryote that is prone to gene amplification and adapts to cytotoxic host environments by stress-induced and chaperone-dependent stage differentiation. Combining phylogenetic and comparative analyses of trypanosomatid genomes, draft genome of Paratrypanosoma and recently published genome sequences of 204 L. donovani field isolates, we gained unique insight into the evolutionary dynamics of the Leishmania HSP70 protein family. We provide evidence for (i) significant evolutionary expansion of this protein family in Leishmania through gene amplification and functional specialization of highly conserved canonical HSP70 members, (ii) evolution of trypanosomatid-specific, non-canonical family members that likely gained ATPase-independent functions, and (iii) loss of one atypical HSP70 member in the Trypanosoma genus. Finally, we reveal considerable copy number variation of canonical cytoplasmic HSP70 in highly related L. donovani field isolates, thus identifying this locus as a potential hot spot of environment-genotype interaction. Our data draw a complex picture of the genetic history of HSP70 in trypanosomatids that is driven by the remarkable plasticity of the Leishmania genome to undergo massive intra-chromosomal gene amplification to compensate for the absence of regulated transcriptional control in these parasites. |
| Databáze: | OpenAIRE |
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