Application of Response Surface Analysis to Evaluate the Effect of Concentrations of Ammonia and Propionic Acid on Acetate-Utilizing Methanogenesis
Autor: | Trong Hoan Do, Su In Kim, Seung Gu Shin, Joonyeob Lee, Seokhwan Hwang |
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Jazyk: | angličtina |
Rok vydání: | 2019 |
Předmět: |
0106 biological sciences
anaerobic digestion Control and Optimization Central composite design Methanogenesis Energy Engineering and Power Technology 010501 environmental sciences 01 natural sciences lcsh:Technology inhibition response surface analysis real-time PCR Ammonia chemistry.chemical_compound Biogas 010608 biotechnology Food science Electrical and Electronic Engineering Engineering (miscellaneous) 0105 earth and related environmental sciences Methanosarcinaceae chemistry.chemical_classification biology Renewable Energy Sustainability and the Environment lcsh:T biology.organism_classification Methanogen Anaerobic digestion chemistry Propionate Energy (miscellaneous) |
Zdroj: | Energies, Vol 12, Iss 17, p 3394 (2019) Energies; Volume 12; Issue 17; Pages: 3394 |
ISSN: | 1996-1073 |
Popis: | Ammonia and propionate are known inhibitors of anaerobic methanogenesis at higher concentrations, and are likely to coexist in digesters treating high-strength wastewater. Therefore, this study was conducted to assess the effects of ammonia and propionate on acetate-utilizing methanogenesis when they coexist. Response surface analysis with face-centered central composite design was used to explore the total ammonia nitrogen (TAN) level of 2–5 g/L and the propionate level of 2–8 g/L in acetate-fed batch incubation. Two models were successfully derived to estimate the lag period and the methane yield in response to the concentrations of the two chemicals. The lag period was affected by both inhibitors, with clues obtained of a synergistic effect at a higher concentration rage ([TAN] > 3.5 g/L and [propionate] > 5 g/L). The methane yield was also affected by the two inhibitors; between the two, it was more significantly dependent on the TAN concentration than on the propionate concentration. Real-time PCR showed that Methanosarcinaceae was the major methanogen group in this system. The results of this study improve our understanding of the inhibition of biogas reactors. |
Databáze: | OpenAIRE |
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