DNA methylation and mRNA expression of COL6A3 in antler mesenchyme of female and male reindeer
Autor: | Wei‑Shi Liu, He-Ping Li, Jian‑Cheng Zhai, Ya‑Jie Yin, Sheng‑Nan Wang, Yan‑Ling Xia, Ruo‑Bing Han, Qiang‑Hui Wang |
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Rok vydání: | 2019 |
Předmět: |
Male
0106 biological sciences 0301 basic medicine animal structures Sequence analysis Mesenchyme Bisulfite sequencing Antlers Biology 01 natural sciences Biochemistry Andrology 03 medical and health sciences Genetics medicine Animals RNA Messenger Molecular Biology Gene Mesenchymal Stem Cells Promoter Methylation DNA Methylation Antler 030104 developmental biology medicine.anatomical_structure DNA methylation Female Collagen Reindeer 010606 plant biology & botany |
Zdroj: | Genes & Genomics. 41:1007-1013 |
ISSN: | 2092-9293 1976-9571 |
Popis: | Reindeer is the only deer species that both male and female produce antlers, which provides a particularly interesting case in studying the differences between antlers of the two sexes. Alpha 3(VI) Collagen Gene (COL6A3), forms a microfibrillar network associated with the structural integrity and biomechanical properties, has been found to be one of the differentially expressed genes in antler mesenchyme of female and male reindeer. The promoter sequence of reindeer COL6A3 gene was obtained using the cloning technology and analyzed by the bioinformatics methods. Bisulfite sequencing PCR (BSP) was used to detect the methylation status of the COL6A3 promoter in reindeer antler mesenchyme. Real-time quantitative PCR was used to detect COL6A3 expression in the antler mesenchyme of female and male reindeer. Sequence analysis revealed that the reindeer COL6A3 partial promoter sequence was 983 bp including the possible promoter region at + 105 bp to + 155 bp. Homology and phylogenetic analysis indicated that the COL6A3 promoter of reindeer had the closest genetic distance with Bos taurus, Capra hircus and Ovis aries. BSP results indicated that the methylation level of COL6A3 promoter in the female reindeer antler mesenchyme was significantly higher than in the male. Correlating with increased methylation status, we also found that COL6A3 mRNA expression in female reindeer antler mesenchyme was significantly lower than in the male. The higher methylation level of the COL6A3 gene in female reindeer antler mesenchyme coincides with decreased COL6A3 mRNA expression, thereby affecting the transposon silencing mechanism and possibly contributing to apparent differences of antlers in female and male reindeer. |
Databáze: | OpenAIRE |
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