Potential of Pseudoshikonin I Isolated from Lithospermi Radix as Inhibitors of MMPs in IL-1β-Induced SW1353 Cells
| Autor: | Je Hun Choi, Young-Seob Lee, Jong-Gil Choi, Ye-Joo Lee, Geum-Soog Kim, Dae Young Lee, Soo-Im Choi, Se Hee Han, Seung Eun Lee |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Matrix Metalloproteinase 3 Magnetic Resonance Spectroscopy Cell Survival nuclear magnetic resonance (NMR) Matrix metalloproteinase inhibitor Chemical structure Interleukin-1beta Matrix Metalloproteinase Inhibitors DEPT Matrix metalloproteinase Lithospermi radix pseudoshikonin I matrix-metalloproteinase (MMPs) Article Catalysis lcsh:Chemistry Inorganic Chemistry 03 medical and health sciences Cell Line Tumor Matrix Metalloproteinase 13 Humans Physical and Theoretical Chemistry lcsh:QH301-705.5 Molecular Biology Spectroscopy Tissue Inhibitor of Metalloproteinase-2 Chromatography Molecular Structure Chemistry Lithospermum Organic Chemistry Interleukin General Medicine Nuclear magnetic resonance spectroscopy Matrix Metalloproteinases Computer Science Applications 030104 developmental biology lcsh:Biology (General) lcsh:QD1-999 Matrix Metalloproteinase 9 Biochemistry Cell culture Matrix Metalloproteinase 1 |
| Zdroj: | International Journal of Molecular Sciences; Volume 17; Issue 8; Pages: 1350 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES(17): 8 International Journal of Molecular Sciences International Journal of Molecular Sciences, Vol 17, Iss 8, p 1350 (2016) |
| ISSN: | 1422-0067 |
| DOI: | 10.3390/ijms17081350 |
| Popis: | Pseudoshikonin I, the new bioactive constituent of Lithospermi radix, was isolated from this methanol extract by employing reverse-phase medium-pressure liquid chromatography (MPLC) using acetonitrile/water solvent system as eluents. The chemical structure was determined based on spectroscopic techniques, including 1D NMR (H-1, C-13, DEPT), 2D NMR (gCOSY, gHMBC, gHMQC), and QTOF/MS data. In this study, we demonstrated the effect of pseudoshikonin I on matrix-metalloproteinase (MMPs) activation and expression in interleukin (IL)-1 beta-induced SW1353 chondrosarcoma cells. MMPs are considered important for the maintenance of the extracellular matrix. Following treatment with PS, active MMP-1, -2, -3, -9, -13 and TIMP-2 were quantified in the SW1353 cell culture supernatants using a commercially available ELISA kit. The mRNA expression of MMPs in SW1353 cells was measured by RT-PCR. Pseudoshikonin I treatment effectively protected the activation on all tested MMPs in a dose-dependent manner. TIMP-2 mRNA expression was significantly upregulated by pseudoshikonin I treatment. Overall, we elucidated the inhibitory effect of pseudoshikonin on MMPs, and we suggest its use as a potential novel anti-osteoarthritis agent. |
| Databáze: | OpenAIRE |
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