Curcumin Prevents Aflatoxin B1 Hepatoxicity by Inhibition of Cytochrome P450 Isozymes in Chick Liver
| Autor: | Jie Liu, Desheng Qi, Shahid Ali Rajput, Christopher Steven Krumm, Chang-Qin Gu, Lv-Hui Sun, Ming-Kun Zhu, Ling Zhao, Jiao Guo, Ni-Ya Zhang, Ming Qi |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Male Aflatoxin chicks Antioxidant Aflatoxin B1 Curcumin Health Toxicology and Mutagenesis medicine.medical_treatment lcsh:Medicine CYP450 Pharmacology Biology Toxicology Article 03 medical and health sciences chemistry.chemical_compound DNA Adducts Cytochrome P-450 Enzyme System curcumin aflatoxin B1 AFBO–DNA medicine Animals Cytochrome P-450 Enzyme Inhibitors RNA Messenger CYP2A6 Liver injury 030102 biochemistry & molecular biology lcsh:R CYP1A2 Glutathione Malondialdehyde medicine.disease Isoenzymes 030104 developmental biology chemistry Biochemistry Liver biology.protein Carcinogens Microsomes Liver Chemical and Drug Induced Liver Injury Chickens Peroxidase |
| Zdroj: | Toxins Toxins, Vol 8, Iss 11, p 327 (2016) Toxins; Volume 8; Issue 11; Pages: 327 |
| ISSN: | 2072-6651 |
| Popis: | This study was designed to establish if Curcumin (CM) alleviates Aflatoxin B1 (AFB1)-induced hepatotoxic effects and to determine whether alteration of the expression of cytochrome P450 (CYP450) isozymes is involved in the regulation of these effects in chick liver. One-day-old male broilers (n = 120) were divided into four groups and used in a two by two factorial trial in which the main factors included supplementing AFB1 (< 5 vs. 100 μg/kg) and CM (0 vs. 150 mg/kg) in a corn/soybean-based diet. Administration of AFB1 induced liver injury, significantly decreasing albumin and total protein concentrations and increasing alanine aminotransferase and aspartate aminotransferase activities in serum, and induced hepatic histological lesions at week 2. AFB1 also significantly decreased hepatic glutathione peroxidase, catalase, and glutathione levels, while increasing malondialdehyde, 8-hydroxydeoxyguanosine, and exo-AFB1-8,9-epoxide (AFBO)-DNA concentrations. In addition, the mRNA and/or activity of enzymes responsible for the bioactivation of AFB1 into AFBO—including CYP1A1, CYP1A2, CYP2A6, and CYP3A4—were significantly induced in liver microsomes after 2-week exposure to AFB1. These alterations induced by AFB1 were prevented by CM supplementation. Conclusively, dietary CM protected chicks from AFB1-induced liver injury, potentially through the synergistic actions of increased antioxidant capacities and inhibition of the pivotal CYP450 isozyme-mediated activation of AFB1 to toxic AFBO. |
| Databáze: | OpenAIRE |
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