Low-Stringency Single-Specific-Primer PCR as a Tool for Detection of Mutations in the rpoB Gene of Rifampin-Resistant Mycobacterium tuberculosis
| Autor: | Silvana Spíndola de Miranda, João Bosco Pesquero, Claudio José Augusto, Kátia M. Costa, Wania da Silva Carvalho, José G. V. C. Araújo, Maria Carmen Aires Gomes, Jorge L. Pesquero |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2003 |
| Předmět: |
Microbiology (medical)
Sequence analysis Molecular Sequence Data Drug resistance Microbial Sensitivity Tests Biology Polymerase Chain Reaction DNA sequencing Microbiology law.invention Mycobacterium tuberculosis Species Specificity law Drug Resistance Bacterial medicine polycyclic compounds Humans Antibiotics Antitubercular Tuberculosis Pulmonary Polymerase chain reaction Antibacterial agent DNA Primers Base Sequence Mycobacteriology and Aerobic Actinomycetes DNA-Directed RNA Polymerases Sequence Analysis DNA biochemical phenomena metabolism and nutrition biology.organism_classification rpoB Mutation Electrophoresis Polyacrylamide Gel Rifampin Rifampicin medicine.drug |
| Popis: | By the low-stringency single-specific-primer PCR technique, a highly sensitive and rapid method for diagnosis of rifampin resistance in Mycobacterium tuberculosis was established. Seven rifampin-resistant and five rifampin-susceptible specimens were analyzed. Rifampin resistance was determined by MIC measurement. A complex electrophoretic pattern consisting of many bands was obtained for both susceptible and rifampin-resistant isolates. The same pattern was obtained for all of the susceptible specimens, but differences between resistant and susceptible isolates were found. DNA sequencing showed that a particular mutation produces a specific electrophoretic pattern. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|