Experimental and visual research on the microbial induced carbonate precipitation by Pseudomonas aeruginosa
| Autor: | Tao Huang, Yang Bai, Xu-jing Guo, Yun-zhen Li |
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| Rok vydání: | 2017 |
| Předmět: |
Biomineralization
0301 basic medicine In situ Calcite MICP Precipitation (chemistry) 030106 microbiology Biophysics Biofilm Mineralogy biochemical phenomena metabolism and nutrition Biology Confocal scanning microscopy Applied Microbiology and Biotechnology 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology Calcium carbonate chemistry Chemical engineering Biofilms Carbonate Original Article |
| Zdroj: | AMB Express |
| ISSN: | 2191-0855 |
| DOI: | 10.1186/s13568-017-0358-5 |
| Popis: | Microbial induced carbonate precipitation (MICP) is a common occurrence of geochemistry influences in many fields, such as biological, geographical, and engineering systems. However, the processes that control interactions between carbonate biomineralization and biofilm properties are poorly understood. We develop a method for real time, in situ and nondestructive imaging with confocal scanning microscopy. This method provides a possible way to observe biomineralization process and the morphology of biomineralized deposits within biofilms. We use this method to show calcite biominerals produced by Pseudomonas aeruginosa biofilms which extremely change biofilm structures. The distribution of calcite precipitation produced in situ biomineralization is highly heterogeneous in biofilms and also to occur primarily on the bottom of biofilms. It is distinct from those usual expectations that mineral started to precipitate from surface of biofilm. Our results reveal that biomineralization plays a comprehensive regulation function on biofilm architecture and properties. Electronic supplementary material The online version of this article (doi:10.1186/s13568-017-0358-5) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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