Neuronal ribosomes exhibit dynamic and context-dependent exchange of ribosomal proteins
| Autor: | Anja Staab, Veronica Villeri, Kristina Desch, Erin M. Schuman, Claudia M Fusco, Julian David Langer, Ivy C. W. Chan, Aline Ricarda Dörrbaum, Mantian Wang, Eleanor Vail |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Male
metabolism [Ribosomal Proteins] Neuropil General Physics and Astronomy Ribosome biogenesis metabolism [Axons] Ribosome Rats Sprague-Dawley 0302 clinical medicine Protein biosynthesis Ribosome profiling Cells Cultured Neurons 0303 health sciences Multidisciplinary Chemistry Translation (biology) metabolism [Neurites] genetics [Ribosomes] Cell biology medicine.anatomical_structure metabolism [Neurons] Cell polarity Female genetics [Ribosomal Proteins] ddc:500 Ribosomal Proteins Science metabolism [Neuropil] Molecular neuroscience metabolism [RNA Messenger] Article General Biochemistry Genetics and Molecular Biology 03 medical and health sciences genetics [RNA Messenger] Ribosomal protein Neurites medicine Animals RNA Messenger 030304 developmental biology General Chemistry metabolism [Ribosomes] Axons Rats nervous system Cytoplasm Protein Biosynthesis Ribosomes 030217 neurology & neurosurgery |
| Zdroj: | Nature Communications 12(1), 6127 (2021). doi:10.1038/s41467-021-26365-x Nat. Commun. Nature Communications, Vol 12, Iss 1, Pp 1-14 (2021) Nature Communications Nature Communications (Nat Commun) |
| Popis: | Owing to their morphological complexity and dense network connections, neurons modify their proteomes locally, using mRNAs and ribosomes present in the neuropil (tissue enriched for dendrites and axons). Although ribosome biogenesis largely takes place in the nucleus and perinuclear region, neuronal ribosomal protein (RP) mRNAs have been frequently detected remotely, in dendrites and axons. Here, using imaging and ribosome profiling, we directly detected the RP mRNAs and their translation in the neuropil. Combining brief metabolic labeling with mass spectrometry, we found that a group of RPs rapidly associated with translating ribosomes in the cytoplasm and that this incorporation was independent of canonical ribosome biogenesis. Moreover, the incorporation probability of some RPs was regulated by location (neurites vs. cell bodies) and changes in the cellular environment (following oxidative stress). Our results suggest new mechanisms for the local activation, repair and/or specialization of the translational machinery within neuronal processes, potentially allowing neuronal synapses a rapid means to regulate local protein synthesis. Ribosomes, assembled in the nucleus and perinuclear region, are thought to remain invariant throughout their lifetime. Here the authors document the dynamic association or exchange of ribosomal proteins within mature ribosomes of neuronal cells. |
| Databáze: | OpenAIRE |
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