Genetic and physical maps of jerker (Espn(je)) on mouse chromosome 4
| Autor: | Torrance Jackson, James W. Thomas, Eric D. Green, Konrad Noben-Trauth |
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| Rok vydání: | 2002 |
| Předmět: |
Genetic Markers
Chromosomes Artificial Bacterial Biophysics Locus (genetics) Biology Physical Maps Biochemistry Chromosomes Mice Mice Neurologic Mutants Homologous chromosome Animals Humans Molecular Biology Gene Actin Genetics Bacterial artificial chromosome Contig Microfilament Proteins Chromosome Mapping Cell Biology Physical Chromosome Mapping Molecular biology Chromosome 4 Chromosomes Human Pair 1 |
| Zdroj: | Biochemical and biophysical research communications. 296(5) |
| ISSN: | 0006-291X |
| Popis: | The jerker mutation causes degeneration of cochlea and vestibular sensoryhair cells in mice. A frame-shift mutation in the actin bundling gene Espin (Espn) leads to hair bundle defects bydisrupting the actin filament assemblyin stereocilia. Previously , jerker was mapped to distal mouse chromosome 4. Here, analyzing 2536 informative meioses derived from two intersubspecific intercrosses, we localize jerker to a 0:51 � 0:14 cM interval on chromosome 4. The following order and distances of genes and markers were determined: D4Mit180—0:44 � 0:13 cM—Hes2, Espn je —0:08 � 0:06 cM—D4Mit356—0:28 � 0: 1c M—D4Mit208. A 300 kb physical bacterial artificial chromosome (BAC) contig was generated containing the Espn je locus. The human homologous region maps to 1p36.31. We present a detailed high-resolution genetic and physical map of markers located at distal chromosome 4 and demonstrate concordance of Espn with jerker. 2002 Elsevier Science (USA). All rights reserved. |
| Databáze: | OpenAIRE |
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