Heavy metal hypertolerant eukaryotic aldehyde dehydrogenase isolated from metal contaminated soil by metatranscriptomics approach
| Autor: | M. Sudhakara Reddy, Rajiv Yadav, Roland Marmeisse, Arkadeep Mukherjee, Laurence Fraissinet-Tachet |
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| Přispěvatelé: | Laboratoire d'Ecologie Microbienne - UMR 5557 (LEM), Centre National de la Recherche Scientifique (CNRS)-Ecole Nationale Vétérinaire de Lyon (ENVL)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Recherche Agronomique (INRA)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS), Department of Biotechnology, University of Tehran-University College of Science, Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Ecole Nationale Vétérinaire de Lyon (ENVL), Council of Scientific and Industrial Research, India, Govt. of India 38(1425)/16/EMR-II, Indo-French Centre for the Promotion of Advanced Research (IFCPAR) 4709-1 |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Eukaryotes Sequence analysis Mutant Yeast complementation Sequence Homology Aldehyde dehydrogenase Biochemistry Soil 03 medical and health sciences Metals Heavy Complementary DNA Soil Pollutants Amino Acid Sequence Gene Ecosystem Phylogeny Soil Microbiology ComputingMilieux_MISCELLANEOUS Metatranscriptomics Copper tolerance 030102 biochemistry & molecular biology biology Chemistry cDNA library Gene Expression Profiling Eukaryota General Medicine Aldehyde Dehydrogenase Soil contamination Yeast Biodegradation Environmental 030104 developmental biology 13. Climate action biology.protein [SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology Transcriptome Heavy metal tolerance Copper |
| Zdroj: | Biochimie Biochimie, Elsevier, 2019, 160, pp.183-192. ⟨10.1016/j.biochi.2019.03.010⟩ |
| ISSN: | 0300-9084 |
| DOI: | 10.1016/j.biochi.2019.03.010⟩ |
| Popis: | International audience; Constant addition of heavy metal pollutants in soil resulting from anthropogenic activities can prove detrimental to both macro and micro life forms inhabiting the ecosystem. The potential functional roles of eukaryotic microbes in such environment were explored in this study by metatranscriptomics approach. Sized eukaryotic cDNA libraries, library A (1 kb) were constructed from the soil RNA and screened for copper (Cu) tolerance genes by using copper sensitive yeast mutant strain cup1(Delta). Screening of the cDNA libraries yielded different clones capable of growing in Cu amended medium. In the present investigation, one of the transcripts PLCc38 from the library C was characterized and tested for its ability to tolerate different heavy metals by using metal sensitive yeast mutants. Sequence analysis PLCc38 showed homology with aldehyde dehydrogenase (ALDH) and capable of tolerating high concentrations of Cu (150-1000 mu M). Aldeyde dehydrogenases are stress response enzymes capable of eliminating toxic levels of aldehydes generated due to abiotic environmental stresses. The cDNA PLCc38 also provided tolerance to wide range of Cd (40-100 mu M), Zn (10-13 mM) and Co (2-50 mM) concentrations. Oxidative stress tolerance potential of PLCc38 was also confirmed in presence of different concentrations of H2O2. This study proves that PLCc38 is a potent gene associated with metal tolerance which could be used to revegetate heavy metal polluted soil or as a biomarker for detection of metal contamination. (C) 2019 Elsevier B. V. and Societe Francaise de Biochimie et Biologie Moleculaire (SFBBM). |
| Databáze: | OpenAIRE |
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