Comparison of different methods used in drugs of abuse for sample validity testing including pH methods, specific gravity methods, TECO™ Drug Adulteration Test Strip and oxidant assay
| Autor: | Deirdre Holmes, Ashraf Mina, Emily Bottero, Santiago Vazquez, Leah McNeice, Kristi-Lee Fletcher, Shanmugam Banukumar, Taveet Sinanian, John Stathopoulos |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
021110 strategic
defence & security studies Drugs of abuse Chromatography 040301 veterinary sciences business.industry ph Sample (material) 0211 other engineering and technologies sample validity Medicine (miscellaneous) oxidant assay Drug Adulteration 04 agricultural and veterinary sciences 02 engineering and technology drug adulteration Education Test (assessment) 0403 veterinary science Medical Laboratory Technology Medical technology Medicine R855-855.5 business specific gravity Specific gravity |
| Zdroj: | Advances in Laboratory Medicine, Vol 2, Iss 4, Pp 550-557 (2021) |
| Popis: | Objectives In the absence of sample validity testing, a healthcare provider may fail to identify a patient’s adulteration of their urine sample. This study compared different methods for specific gravity (SG), pH, TECO™ Drug Adulteration Test Strip (dipstick) and oxidant assay to explain the differences and also make an informative decision on method selection. Methods Creatinine, SG and pH measurements are essential in sample validity testing. SG and pH automated chemical methods are compared against pH meter method, SG refractometer and dipstick method. Also, oxidant assay was compared against dipstick method. Results SG chemical method agreement with refractometer is 81.9% and with dipstick method is 64.7%. The refractometer method agreement with dipstick method is 66.1%. pH chemical method agreement with pH Meter method is 74.3% and with dipstick method is 81.4%. pH meter method agreement is 85.7% with dipstick method. Results were analysed using Deming regression analysis and F-test. SG chemical method correlated better with refractometer than the dipstick method. Oxidant assay correlated well with dipstick method in detecting adulterants such as pyridinium chlorochromate, nitrite and bleach. Conclusions Varying degrees of differences were seen in the SG and pH measurements. These differences were both method and instrument dependent. The automated chemical methods are recommended alongside oxidant assay for consistency, accuracy and faster turn-around time as part of sample validity testing for drugs of abuse. |
| Databáze: | OpenAIRE |
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