Inflammatory effects of in vivo glycated albumin from cardiovascular patients

Autor: Beatriz Paradela-Dobarro, Ezequiel Álvarez, Mercedes González-Peteiro, Alfonso Varela-Román, José Ramón González-Juanatey, Adriana Rozados-Luis, Susana B. Bravo, Javier García-Seara
Rok vydání: 2018
Předmět:
0301 basic medicine
Glycation End Products
Advanced
medicine.medical_specialty
In vivo glycated albumin
Mononuclear cells adhesion
RM1-950
Peripheral blood mononuclear cell
Umbilical vein
03 medical and health sciences
Young Adult
0302 clinical medicine
In vivo
Glycation
Internal medicine
medicine
Cell Adhesion
Human Umbilical Vein Endothelial Cells
Humans
Glycated Serum Albumin
Endothelial dysfunction
Vascular endothelial dysfunction
Serum Albumin
Pharmacology
Heart Failure
Inflammation
Chemistry
Cell adhesion molecule
Albumin
General Medicine
Human endothelial cells
Middle Aged
medicine.disease
Human serum albumin
Up-Regulation
030104 developmental biology
Endocrinology
030220 oncology & carcinogenesis
Spectrometry
Mass
Matrix-Assisted Laser Desorption-Ionization
Leukocytes
Mononuclear
Therapeutics. Pharmacology
Cell Adhesion Molecules
medicine.drug
Peptide mapping of glycomodified proteins
Zdroj: Biomedicine & Pharmacotherapy, Vol 113, Iss, Pp-(2019)
ISSN: 1950-6007
Popis: Objectives Characterization of the type of glycation found in circulating proteins from cardiovascular patients in comparison with healthy control subjects and to explore the pathophysiological molecular effects of these glycomodified proteins on human umbilical vein endothelial cells (HUVEC) in culture. Methods Human serum albumin pools from 10 subjects each, of patients with heart failure (HF) presenting high or low glycation levels, and from healthy subjects were isolated and purified. The glycation levels of these pools were characterized by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and compared between them. Analysis of endothelial dysfunction after the treatment of HUVEC with the pools was made by mRNA expression of adhesion molecules and by functional adhesion of mononuclear cells to HUVEC monolayers. Results Specific characterization of post-transductional modifications (advanced glycation end products) in high and low glycated albumins from patients was made in comparison with healthy subjects. Albumins from patients were able, at very low concentrations (12.5 μg/mL), to significantly up-regulate (˜0.2 – 2 fold) the gene expression of adhesion molecules in HUVEC. At the functional level, the albumin from patients with high glycation levels (at 12.5 and 25 μg/mL) significantly enhanced (˜10%) the adhesion of mononuclear cells to HUVEC. Conclusions Differences in the glycomodification of albumin from HF patients were found and specifically characterized in comparison with albumin from healthy subjects. Functionally, in vivo glycated albumin in patients with HF induced an increase in adhesion molecules expression on HUVEC, which supported an increase in peripheral blood mononuclear cells adhesion to endothelial cells.
Databáze: OpenAIRE
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