Tumor size-independence of telomere length indicates an aggressive feature of HCC
| Autor: | Tomoki Nakajima, Masahito Minami, Hiroyuki Kimura, Keizo Kagawa, Takeshi Okanoue, Tadashi Watanabe, Satoru Sekoguchi, Taichirou Nishikawa, Yoshito Itoh, Hidetaka Takashima, Tatsuo Katagishi, Michihisa Moriguchi |
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| Rok vydání: | 2004 |
| Předmět: |
Male
Telomerase Carcinoma Hepatocellular Stromal cell Biophysics In situ hybridization Biology Sensitivity and Specificity Severity of Illness Index Biochemistry medicine Carcinoma Humans Molecular Biology In Situ Hybridization Fluorescence Cell Proliferation Neoplasm Staging Liver Neoplasms Reproducibility of Results Histology Cell Biology Middle Aged Telomere medicine.disease Molecular biology Hepatocellular carcinoma Cancer cell Female |
| Zdroj: | Biochemical and Biophysical Research Communications. 325:1131-1135 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/j.bbrc.2004.10.152 |
| Popis: | Using quantitative fluorescence in situ hybridization (Q-FISH), the average telomere length of hepatoma cells was assessed by the average telomeric signal intensity of cancer cells relative to that of stromal cells. We demonstrated first the applicability of Q-FISH for tissue sections by comparing Q-FISH and Southern blotting results. Tumors less than 50 mm in diameter and with a relative telomeric intensity of less than 0.6 were categorized as group A and the remainder as group B. In group A, the telomere length correlated negatively with tumor size, whereas in group B there was no correlation. Compared with the group A tumors, the group B tumors were of significantly more advanced stage, showed higher telomerase and proliferative activities, and exhibited less differentiated histology. Therefore, we considered that a lack of correlation between telomere length and tumor size, namely, size-independence of telomere length, is associated with unfavorable clinicopathological features of hepatocellular carcinomas. |
| Databáze: | OpenAIRE |
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