Therapeutic Effect of Obestatin in the Course of Cerulein-Induced Acute Pancreatitis
| Autor: | Zygmunt Warzecha, Artur Dembiński, Beata Kusnierz-Cabala, Piotr Ceranowicz, Romana Tomaszewska, Jakub Bukowczan, Jakub Cieszkowski |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Male medicine.medical_specialty Time Factors Endocrinology Diabetes and Metabolism Interleukin-1beta Severity of Illness Index Drug Administration Schedule Proinflammatory cytokine 03 medical and health sciences 0302 clinical medicine Endocrinology Internal medicine Edema Internal Medicine medicine Animals Regeneration Rats Wistar Pancreas Ceruletide Hepatology business.industry Therapeutic effect DNA Obestatin medicine.disease Ghrelin 030104 developmental biology medicine.anatomical_structure Treatment Outcome Pancreatitis Regional Blood Flow 030220 oncology & carcinogenesis Acute Disease Acute pancreatitis medicine.symptom business |
| Zdroj: | Pancreas. 45(5) |
| ISSN: | 1536-4828 |
| Popis: | Objective: The aim of this study was to determine the impact of obestatin therapy on the course of cerulein-induced pancreatitis. Methods: Acute pancreatitis was induced by cerulein given intraperitoneally 5 times with 1 hour intervals at the dose of 50 mu g/kg per dose. Obestatin was administered twice a day at the dose of 8 nmol/kg per dose, starting the first dose 24 hours after the last injection of cerulein. Severity of acute pancreatitis (AP) was examined at 0 hour or 1, 2, 3, 5, 7, and 10 days after the last injection of cerulein. Results: Administration of cerulein led to development of acute edematous pancreatitis in all rats, and maximal severity of this disease was observed 24 hours after induction of pancreatitis. Treatment with obestatin reduced morphological signs of pancreatic damage (pancreatic edema, leukocyte infiltration, vacuolization of acinar cells) and led to earlier regeneration of the pancreas. Biochemical indexes of severity of pancreatitis such as serum activity of pancreatic digestive enzymes were significantly reduced in animals treated with obestatin. These effects were accompanied by increase in pancreatic DNA synthesis and decrease in serum level of proinflammatory interleukin 1 beta. In addition, administration of obestatin improved pancreatic blood flow in rats with AP. Conclusions: Treatment with exogenous obestatin reduces severity of AP and accelerates pancreatic recovery. |
| Databáze: | OpenAIRE |
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