Lactobacillus delbrueckii UFV-H2b20 induces type 1 cytokine production by mouse cells in vitro and in vivo
| Autor: | Jacques Robert Nicoli, Enio C. Vieira, Mariana Gontijo Ramos, Liliane Martins dos Santos, Leda Quercia Vieira, Luís Carlos Crocco Afonso, A.C.P. Rodrigues, Elisabeth Neumann |
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| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
Physiology
medicine.medical_treatment Immunology Biophysics Biology Biochemistry Microbiology Interferon-gamma Mice In vivo Lactobacillus IFN-γ medicine Escherichia coli Animals Germ-Free Life General Pharmacology Toxicology and Pharmaceutics lcsh:QH301-705.5 lcsh:R5-920 Lactobacillus delbrueckii Mice Inbred C3H Tumor Necrosis Factor-alpha General Neuroscience Probiotics Macrophages Cell Biology General Medicine biology.organism_classification Interleukin-12 In vitro Cytokine lcsh:Biology (General) IL-12 Macrophages Peritoneal Cytokines lcsh:Medicine (General) |
| Zdroj: | Brazilian Journal of Medical and Biological Research v.42 n.4 2009 Brazilian Journal of Medical and Biological Research Associação Brasileira de Divulgação Científica (ABDC) instacron:ABDC Brazilian Journal of Medical and Biological Research, Vol 42, Iss 4, Pp 358-367 (2009) Brazilian Journal of Medical and Biological Research, Volume: 42, Issue: 4, Pages: 358-367, Published: APR 2009 Repositório Institucional da UFOP Universidade Federal de Ouro Preto (UFOP) instacron:UFOP ResearcherID |
| Popis: | Lactobacillus delbrueckii UFV-H2b20 has been shown to increase clearance of bacteria injected into the blood of germ-free mice. Moreover, it induces the production of type 1 cytokines by human peripheral mononuclear cells. The objective of the present study was to investigate the production of inflammatory cytokines [interleukin-12 (IL-12 p40), tumor necrosis factor-α (TNF-α), and interferon-γ (IFN-γ)] triggered in vitro by live, heat-killed or lysozyme-treated L. delbrueckii UFV-H2b20 and in vivo by a live preparation. Germ-free, L. delbrueckii-monoassociated and lipopolysaccharide (LPS)-resistant C3H/HeJ mice were used as experimental models. UFV-H2b20 induced the production of IL-12 p40 and TNF-α by peritoneal cells and IFN-γ by spleen cells from germ-free or monoassociated Swiss/NIH mice and LPS-hyporesponsive mice (around 40 ng/mL for IL-12 p40, 200 pg/mL for TNF-α and 10 ng/mL for IFN-γ). Heat treatment of L. delbrueckii did not affect the production of these cytokines. Lysozyme treatment decreased IL-12 p40 production by peritoneal cells from C3H/HeJ mice, but did not affect TNF-α production by these cells or IFN-γ production by spleen cells from the same mouse strain. TNF-α production by peritoneal cells from Swiss/NIH L. delbrueckii-monoassociated mice was inhibited by lysozyme treatment. When testing IL-12 p40 and IFN-γ levels in sera from germ-free or monoassociated Swiss/NIH mice systemically challenged with Escherichia coli we observed that IL-12 p40 was produced at marginally higher levels by monoassociated mice than by germ-free mice (40 vs 60 ng/mL), but IFN-γ was produced earlier and at higher levels by monoassociated mice (monoassociated 4 and 14 ng/mL 4 and 8 h after infection, germfree 0 and 7.5 ng/mL at the same times). These results show that L. delbrueckii UFV-H2b20 stimulates the production of type 1 cytokines in vitro and in vivo, therefore suggesting that L. delbrueckii might have adjuvant properties in infection in which these cytokines play a major role. |
| Databáze: | OpenAIRE |
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