The antigenic anatomy of SARS-CoV-2 receptor binding domain

Autor: Neil G. Paterson, Rita E. Chen, Elizabeth E. Fry, Alex Fyfe, Christina Dold, Chang Liu, Gavin R. Screaton, Yanchun Peng, Julika Raedecke, Piyada Supasa, Javier Gilbert-Jaramillo, Alexander J. Mentzer, J Slon-Campos, Mark A. Williams, Thomas S. Walter, Guido C. Paesen, Julian C. Knight, Nigel J. Temperton, Donal T. Skelly, Miles W. Carroll, Sunetra Gupta, David I. Stuart, Kuan-Ying A. Huang, Wanwisa Dejnirattisai, Tao Dong, William James, Karen R. Buttigieg, Yuguang Zhao, Tiong Kit Tan, Baoling Ying, David R. Hall, R Levin, A Howe, James Brett Case, C. Alistair Seibert, Michael S. Diamond, Adam L. Bailey, Jingshan Ren, Beibei Wang, Natasha M. Kafai, Felicity K.R. Bertram, Helen M. Ginn, Andrew J. Pollard, Yun Song, Alain Townsend, Helen M. E. Duyvesteyn, Paul Klenerman, Naomi Coombes, Juthathip Mongkolspaya, D. Zhou, Craig Thompson, Cesar Lopez-Camacho, Daniel K. Clare
Rok vydání: 2020
Předmět:
Male
Models
Molecular
Plasma protein binding
medicine.disease_cause
Antibodies
Viral
Epitope
Epitopes
Mice
0302 clinical medicine
Chlorocebus aethiops
Neutralizing antibody
Coronavirus
0303 health sciences
biology
Antibodies
Monoclonal
Medical research
Neutralizing epitope
Research centre
Spike Glycoprotein
Coronavirus
Female
Antibody
Protein Binding
medicine.drug_class
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
Library science
Mice
Transgenic
CHO Cells
Monoclonal antibody
General Biochemistry
Genetics and Molecular Biology
Article
03 medical and health sciences
Light source
Cricetulus
Antigen
Emerging infections
medicine
Animals
Humans
Binding site
Vero Cells
030304 developmental biology
QR355
Immune protection
SARS-CoV-2
COVID-19
Virology
Antibodies
Neutralizing
Protein Structure
Tertiary
HEK293 Cells
Immunome
biology.protein
Binding Sites
Antibody
030217 neurology & neurosurgery
Zdroj: Cell
ISSN: 1097-4172
0092-8674
Popis: Antibodies are crucial to immune protection against SARS-CoV-2, with some in emergency use as therapeutics. Here we identify 377 human monoclonal antibodies (mAbs) recognizing the virus spike, and focus mainly on 80 that bind the receptor binding domain (RBD). We devise a competition data driven method to map RBD binding sites. We find that although antibody binding sites are widely dispersed, neutralizing antibody binding is focused, with nearly all highly inhibitory mAbs (IC50
Dejnirattisai et al. present an in-depth study of the human antibody response to SARS-CoV-2 infection. By characterizing 377 human mAbs from recovered COVID-19 patients, and determining 19 protein structures, they construct a map of antibody footprints on the RBD describe in great detail its antigenic anatomy.
Databáze: OpenAIRE
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