Improved NMR Detection of Phospho-Metabolites in a Complex Mixture
| Autor: | Pierre Millard, Guy Lippens, Cyril Charlier, Neil Cox |
|---|---|
| Přispěvatelé: | Toulouse Biotechnology Institute (TBI), Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), European Project: 862179,FLIX, Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) |
| Rok vydání: | 2021 |
| Předmět: |
Magnetic Resonance Spectroscopy
Resolution (mass spectrometry) Chemistry 010401 analytical chemistry Analytical chemistry Complex Mixtures 010402 general chemistry Magnetic Resonance Imaging 01 natural sciences Spectral line Workflow 0104 chemical sciences Analytical Chemistry [CHIM.ANAL]Chemical Sciences/Analytical chemistry [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry Molecular Biology/Biochemistry [q-bio.BM] Absorption (chemistry) Spectroscopy |
| Zdroj: | Analytical Chemistry Analytical Chemistry, American Chemical Society, In press, 93 (11), pp.4818-4824. ⟨10.1021/acs.analchem.0c04056⟩ Analytical Chemistry, American Chemical Society, 2021, 93 (11), pp.4818-4824. ⟨10.1021/acs.analchem.0c04056⟩ Analytical Chemistry, 2021, 93 (11), pp.4818-4824. ⟨10.1021/acs.analchem.0c04056⟩ |
| ISSN: | 1520-6882 0003-2700 8379-8382 |
| DOI: | 10.1021/acs.analchem.0c04056 |
| Popis: | Phosphorylated metabolites are omnipresent in cells, but their analytical characterization faces several technical hurdles. Here, we detail an improved NMR workflow aimed at assigning the high-resolution subspectrum of the phospho-metabolites in a complex mixture. Combining a pure absorption J-resolved spectrum (Pell, A. J.; J. Magn. Reson. 2007, 189 (2), 293-299) with alternate on- and off-switching of the 31P coupling interaction during the t1 evolution with a pure in-phase (PIP) HSQMBC experiment (Castanar, L.; Angew. Chem., Int. Ed. 2014, 53 (32), 8379-8382) without or with total correlation spectroscopy (TOCSY) transfer during the insensitive nuclei enhancement by polarization transfer (INEPT) gives access to selective identification of the individual subspectra of the phosphorylated metabolites. Returning to the initial J-res spectra, we can extract with optimal resolution the full trace for the individual phospho-metabolites, which can then be transposed on the high-resolution quantitative one dimensional spectrum. |
| Databáze: | OpenAIRE |
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