Natural Infection of Baboons by Entamoeba Histolytica Elicits Anti-Gal Lectin Heavy Subunit Iga and Igg Antibodies with Shared Epitope Specificity to That of Humans
| Autor: | Jonathan I. Ravdin, Stanley D. Kosanke, Mie-Jie Zhang, Jaco J. Verweij, David W. Carey, Yasser M. M. El-Dessouky, Roman F. Wolf, Gary L. White, Mohamed D. Abd-Alla |
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| Rok vydání: | 2013 |
| Předmět: |
Male
Galectins Antibodies Protozoan Asymptomatic Epitope Microbiology law.invention Epitopes Entamoeba histolytica law biology.animal parasitic diseases medicine Animals Entamoebiasis biology Monkey Diseases Entamoeba Lectin biology.organism_classification Virology Immunoglobulin A Immunoglobulin G embryonic structures Recombinant DNA biology.protein Female medicine.symptom Antibody Papio Baboon |
| Zdroj: | Journal of the Egyptian Society of Parasitology. 43:723-735 |
| ISSN: | 1110-0583 |
| DOI: | 10.12816/0006429 |
| Popis: | Non-human primates, such as baboons (Papio hamadryas anubis), are natural hosts for Entamoeba species; infections can be asymptomatic or result in invasive lethal disease. It was sought to determine whether following natural infection by Entamoeba. histolytica, baboon anti-amebic antibodies recognized native Gallectin, a recombinant portion of the lectin heavy subunit (designated LC3) and specific heavy subunit epitopes; we compared the specificity of anti-amebic antibodies from baboons to that of humans following asymptomatic E. histolytica infection or cure of amebic liver abscess (ALA). Female baboons (n=54), aged one to three years of age and living in captivity were screened for infection by real time PCR. E. histolytica infection was found in 37 baboons and was associated with serum anti-LC3 IgG (73%) and anti-LC3 IgA (46%) or intestinal anti-Gal-Lectin IgA antibody responses (49%), p0.021 for each compared to that observed with baboons having an E. dispar infection (n=10) or uninfected baboons (n=7). The ELISA OD reading for anti-LC3 or anti-lectin antibodies correlated strongly with the presence of a PCR CT value indicative of E. histolytica infection. In humans with asymptomatic E. histolytica infection or those recently cured of ALA, 63% and 57% had serum anti- LC3 IgA and 65% and 57% had serum anti-LC3 IgG antibodies respectively. Epitope- specific synthetic peptides were used as capture antigens in ELISA; for baboons that possessed anti-LC3 and anti-lectin antibodies, 74% had anti-peptide IgG or IgA antibodies, compared to 86% of asymptomatic humans and 92% of ALA subjects(P0.05). |
| Databáze: | OpenAIRE |
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