Prokaryotic Communities in the Thalassohaline Tuz Lake, Deep Zone, and Kayacik, Kaldirim and Yavsan Salterns (Turkey) Assessed by 16S rRNA Amplicon Sequencing
| Autor: | Antonio Ventosa, Baris Calli, Can Akpolat, Pinar Caglayan, Ana Beatriz Fernández, Meral Birbir |
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| Přispěvatelé: | Universidad de Sevilla. Departamento de Microbiología y Parasitología, European Commission (EC). Fondo Europeo de Desarrollo Regional (FEDER), Ministerio de Ciencia e Innovación (MICIN). España, Junta de Andalucía, Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa. IMAB - Institute for Multidisciplinary Research in Applied Biology, Akpolat, Can, Beatriz Fernandez, Ana, Caglayan, Pinar, Calli, Baris, Birbir, Meral, Ventosa, Antonio |
| Rok vydání: | 2021 |
| Předmět: |
Microbiology (medical)
QH301-705.5 BRINE CURED HIDES Physico-chemical analyses Zoology Microbiology 16S rRNA amplicon sequencing Natronomonas Article 03 medical and health sciences Extremophiles HALOCIN Tuz Lake (Salt Lake) Virology thalassohaline lakes HYPERSALINE ENVIRONMENTS Halomicrobium Biology (General) physico-chemical analyses extremophiles 030304 developmental biology DAMAGE 0303 health sciences metagenomics biology salterns 030306 microbiology biology.organism_classification 16S ribosomal RNA Haloquadratum HALOPHILIC ARCHAEA Thalassohaline lakes Haloarcula Halorhabdus Metagenomics Halorubrum Salterns Archaea MICROBIAL DIVERSITY |
| Zdroj: | idUS. Depósito de Investigación de la Universidad de Sevilla instname Microorganisms, Vol 9, Iss 1525, p 1525 (2021) Microorganisms Academica-e. Repositorio Institucional de la Universidad Pública de Navarra Volume 9 Issue 7 |
| Popis: | Prokaryotic communities and physico-chemical characteristics of 30 brine samples from the thalassohaline Tuz Lake (Salt Lake), Deep Zone, Kayacik, Kaldirim, and Yavsan salterns (Turkey) were analyzed using 16S rRNA amplicon sequencing and standard methods, respectively. Archaea (98.41% of reads) was found to dominate in these habitats in contrast to the domain Bacteria (1.38% of reads). Representatives of the phylum Euryarchaeota were detected as the most predominant, while 59.48% and 1.32% of reads, respectively, were assigned to 18 archaeal genera, 19 bacterial genera, 10 archaeal genera, and one bacterial genus that were determined to be present, with more than 1% sequences in the samples. They were the archaeal genera Haloquadratum, Haloarcula, Halorhabdus, Natronomonas, Halosimplex, Halomicrobium, Halorubrum, Halonotius, Halolamina, Halobacterium, and Salinibacter within the domain Bacteria. The genera Haloquadratum and Halorhabdus were found in all sampling sites. While Haloquadratum, Haloarcula, and Halorhabdus were the most abundant genera, two uncultured Tuz Lake Halobacteria (TLHs) 1 and 2 were detected in high abundance, and an additional uncultured haloarchaeal TLH-3 was found as a minor abundant uncultured taxon. Their future isolation in pure culture would permit us to expand our knowledge on hypersaline thalassohaline habitats, as well as their ecological role and biomedical and biotechnological potential applications. |
| Databáze: | OpenAIRE |
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