Adaptive immune responses against parvovirus B19 in patients with myocardial disease
| Autor: | Susanne Modrow, Jürgen J. Wenzel, Juha Lindner, Uwe Kuehl, Heinz-Peter Schultheiss, Michel Noutsias, Dirk Lassner |
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| Rok vydání: | 2009 |
| Předmět: |
Adult
Male Cardiomyopathies/virology Myocarditis T-Lymphocytes Viral pathogenesis Blotting Western 610 Medizin Cardiomyopathy Enzyme-Linked Immunosorbent Assay Antibodies Viral Polymerase Chain Reaction Serology Parvoviridae Infections Antibodies Viral/blood Immune system Virology Parvovirus B19 Human medicine Humans Aged Parvovirus B19 Human/immunology biology Parvovirus business.industry Dilated cardiomyopathy Middle Aged T-Lymphocytes/immunology Viral Load Immunoglobulin M/blood biology.organism_classification medicine.disease Acquired immune system Parvoviridae Infections/immunology Infectious Diseases Immunoglobulin M Immunoglobulin G/blood Immunoglobulin G DNA Viral Immunology DNA Viral/blood Female Cardiomyopathies business |
| Zdroj: | Journal of Clinical Virology. 44:27-32 |
| ISSN: | 1386-6532 |
| DOI: | 10.1016/j.jcv.2008.09.007 |
| Popis: | Background Parvovirus B19 (B19V)-DNA is frequently detected in endomyocardial biopsies (EMBs) from patients with acute myocarditis (AMC) and dilated cardiomyopathy (DCM), but also in various healthy tissues. The clinical relevance of this DNA-persistence is unclear. Objectives To investigate potential pathogenic influences of B19V-DNA in EMBs, we analyzed B19V-specific adaptive immune responses in AMC/DCM patients and healthy controls. Study design 15 AMC/DCM patients with detectable B19V-DNA in EMBs and 51 controls were analyzed for signs of acute B19V-infections and virus-specific immune responses by PCR, ELISA, Western line, and ELISpot-assays. Results Productive B19V-infection was determined in three patients. Slightly lower levels of B19V-specific T-cells were observed in patients as compared to the controls, no differences were observed in virus-specific serology. Viral DNA-load in EMBs could not be correlated to the number of B19V-specific T-cells. No differences in T-cell response, viremia and/or serological markers indicative for viral pathogenesis were observed in patients with inflammatory cardiomyopathy. Conclusions Discrepancies in B19V-specific adaptive immunity were not observed in AMC/DCM patients as compared to controls. The data indicate that the exclusive detection of B19V-DNA in EMBs is not sufficient to associate B19V with AMC/DCM but should be complemented with additional virological and immunological parameters in further studies. |
| Databáze: | OpenAIRE |
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