Identification of 9α,11β-Prostaglandin F2 in Human Amniotic Fluid and Characterization of Its Production by Human Gestational Tissues
| Autor: | Roberto Romero, Murray D. Mitchell, Maxwell C. Chang, Timothy A. Sato, Hao-Yi Lan, Rachel J. A. Helliwell, Tinnakorn Chaiworapongsa |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Lipopolysaccharides
medicine.medical_specialty Amniotic fluid Placenta Endocrinology Diabetes and Metabolism Clinical Biochemistry Uterus Prostaglandin Context (language use) Biology Dinoprost Biochemistry chemistry.chemical_compound Endocrinology Pregnancy Internal medicine Decidua medicine Humans Amnion Prostaglandin D2 Tumor Necrosis Factor-alpha Biochemistry (medical) Chorion Amniotic Fluid medicine.anatomical_structure chemistry Eicosanoid Gestation Female lipids (amino acids peptides and proteins) Interleukin-1 |
| Zdroj: | The Journal of Clinical Endocrinology & Metabolism. 90:4244-4248 |
| ISSN: | 1945-7197 0021-972X |
| DOI: | 10.1210/jc.2004-2496 |
| Popis: | Context: 9α,11β-Prostaglandin F2 (9α,11β-PGF2) can contract uterine smooth muscle with a potency equal to PGF2α. Its presence in the human uterus and production by human gestational tissues is unknown.Objective: These studies were performed to determine whether the PGD2-derived 9α,11β-PGF2 is both present in human amniotic fluid and synthesized by human gestational tissues and if so, whether labor-related substances could regulate its production.Results: Detectable concentrations of 9α,11β-PGF2 were found in amniotic fluid samples and appeared to increase in late gestation. All gestational tissues studied synthesized 9α,11β-PGF2, with the placenta having the highest basal production rate, followed by the amnion and then the choriodecidua. IL-1β and TNFα caused concentration-dependent increases in 9α,11β-PGF2 production in human amnion and choriodecidual explants. Moreover, treatment of choriodecidual and placental explants with lipopolysaccharide resulted in a significant increase in 9α,11β-PGF2 production rates, reaching a maximum of 13-fold in the choriodecidua. Studies examining the effects of the addition of exogenous PGD2 strongly indicated that the choriodecidua has significant ability to convert PGD2 to 9α,11β-PGF2, whereas the amnion has little.Conclusions: These results demonstrate for the first time that 9α,11β-PGF2 is present in human amniotic fluid and that it is produced by human gestational tissues and up-regulated by bacterial cell wall components and proinflammatory cytokines. We suggest that this prostaglandin may play a part in the mechanisms of human labor at term and preterm. |
| Databáze: | OpenAIRE |
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