Characterization of a Plasmodium falciparium mutant that has deleted the majority of the gametocyte-specific Pf11-1 locus
| Autor: | James Leech, Pietro Alano, Luiz Pereira da Silva, Rick Nelson, Carolyn Petersen, Masamichi Aikawa, Artur Scherf, Denise Mattei, Richard Carter |
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| Předmět: |
Microbiology (medical)
lcsh:Arctic medicine. Tropical medicine lcsh:RC955-962 Mutant Genes Protozoan Plasmodium falciparum lcsh:QR1-502 Protozoan Proteins Locus (genetics) Polymerase Chain Reaction lcsh:Microbiology Chromosomes parasitic diseases Gametocyte Animals Gametogenesis chromosome breakage and healing HRP II Genetics Gene Rearrangement telomere biology Reproduction deletions biology.organism_classification Molecular biology In vitro Telomere gametocyte specific antigen Gene Expression Regulation Chromosome breakage Gene Deletion HRPI |
| Zdroj: | Scopus-Elsevier Memórias do Instituto Oswaldo Cruz, Volume: 87 Supplement 3, Pages: 91-94, Published: 1992 Memórias do Instituto Oswaldo Cruz., Vol 87, Pp 91-94 (1992) |
| Popis: | We identified a gametocyte-specific protein of Plasmodium falciparum called Pf11-1 and provide experimental evidence that this molecule is involved in the emergence of gametes of the infected erythrocyte (gametogenesis). A mutant parasite clone, which has deleted over 90% of the Pf11-1 gene locus, was an important control to establish the gametocyte-specific expression of the Pf11-1. Molecular analysis of the Pf11-1 deletion indicates that it is presumably due to a chromosome breakage with subsequent 'healing' by the addition of telomeric heptanucleotides. Moreover, similar DNA rearrangements are observed in most of the laboratory isolates during asexual propagation in vitro. |
| Databáze: | OpenAIRE |
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