Implantation of 3D Constructs Embedded with Oral Mucosa-Derived Cells Induces Functional Recovery in Rats with Complete Spinal Cord Transection
Autor: | Izhak Michaelevski, Sandu Pitaru, Erez Shor, Anton Sheinin, Shaowei Guo, Shulamit Levenberg, Daniel Offen, Javier Ganz, Ina Arie |
---|---|
Jazyk: | angličtina |
Rok vydání: | 2017 |
Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty Population regenerative medicine Regenerative medicine Neuroprotection Glial scar lcsh:RC321-571 03 medical and health sciences 0302 clinical medicine Tissue engineering stem cells Medicine Oral mucosa education Spinal cord injury lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry Original Research education.field_of_study oral mucosa business.industry General Neuroscience Anatomy medicine.disease spinal cord injury 030104 developmental biology medicine.anatomical_structure tissue engineering Stem cell business 030217 neurology & neurosurgery Neuroscience |
Zdroj: | Frontiers in Neuroscience, Vol 11 (2017) Frontiers in Neuroscience |
Popis: | Spinal cord injury (SCI), involving damaged axons and glial scar tissue, often culminates in irreversible impairments. Achieving substantial recovery following complete spinal cord transection remains an unmet challenge. Here, we report of implantation of an engineered 3D construct embedded with human oral mucosa stem cells (hOMSC) induced to secrete neuroprotective, immunomodulatory, and axonal elongation-associated factors, in a complete spinal cord transection rat model. Rats implanted with induced tissue engineering constructs regained fine motor control, coordination and walking pattern in sharp contrast to the untreated group that remained paralyzed (42 vs. 0%). Immunofluorescence, CLARITY, MRI, and electrophysiological assessments demonstrated a reconnection bridging the injured area, as well as presence of increased number of myelinated axons, neural precursors, and reduced glial scar tissue in recovered animals treated with the induced cell-embedded constructs. Finally, this construct is made of bio-compatible, clinically approved materials and utilizes a safe and easily extractable cell population. The results warrant further research with regards to the effectiveness of this treatment in addressing spinal cord injury. |
Databáze: | OpenAIRE |
Externí odkaz: |