Detection of methandienone (methandrostenolone) and metabolites in horse urine by gas chromatography—mass spectrometry
| Autor: | Heinz-Werner Hagedorn, Rüdiger Schulz, Anita Friedrich |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Doping in Sports
Chromatography Metabolite Methandrostenolone Metandienone Extraction (chemistry) General Chemistry Urine Mass spectrometry Gas Chromatography-Mass Spectrometry chemistry.chemical_compound chemistry medicine Animals Female Epimer Horses Gas chromatography Gas chromatography–mass spectrometry Biotransformation medicine.drug |
| Zdroj: | Journal of Chromatography B: Biomedical Sciences and Applications. 577:195-203 |
| ISSN: | 0378-4347 |
| DOI: | 10.1016/0378-4347(92)80240-q |
| Popis: | The metabolic transformation of methandienone (I) in the horse was investigated. After administration of a commercial drug preparation to a female horse (0.5 mg/kg), urine samples were collected up to 96 h and processed without enzymic hydrolysis. Extraction was performed by a series of solid-liquid and liquid-liquid extractions, thus avoiding laborious purification techniques. For analysis by gas chromatography-mass spectrometry, the extracts were trimethylsilylated. Besides the parent compound I and its C-17 epimer II, three monohydroxylated metabolites were identified: 6 beta-hydroxymethandienone (III), its C-17 epimer (IV) and 16 beta-hydroxymethandienone (V). In addition, three isomers of 6 beta,16-dihydroxymethandienone (VIa-c) were discovered. Apparently, reduction of the delta 4 double bond of 16 beta-hydroxymethandienone (V) in the horse yields 16 beta,17 beta-dihydroxy-17 alpha-methyl-5 beta-androst-1-en-3-one (VII). Reduction of the isomers VIa-c results in the corresponding 6 beta,16,17-trihydroxy-17-methyl-5 beta-androst-1-en-3-ones (VIIIa-c). The data presented here suggest that screening for the isomers of VI and VIII, applying the selected-ion monitoring technique, will be the most successful way of proving methandienone administration to a horse. |
| Databáze: | OpenAIRE |
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