Nanoplasmonic immunosensor for the detection of SCG2, a candidate serum biomarker for the early diagnosis of neurodevelopmental disorder
Autor: | Yun-Ju Sung, Jae-Ran Lee, Jeehun Lee, Narae Jo, Ju-Young Byun, So-Hee Lim, Nam-Soon Kim, Da Yong Lee, Kyoung-Shim Kim, Na-Yoon Lee, Yong-Beom Shin |
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Rok vydání: | 2021 |
Předmět: |
Oncology
medicine.medical_specialty Diagnostic methods Science Diseases Biosensing Techniques Hippocampus Article Medical research Neurodevelopmental disorder Serum biomarkers Internal medicine medicine Animals Humans In patient Child Immunoassay Neurons Gene knockdown Multidisciplinary business.industry medicine.disease Serum samples Publisher Correction Rats Early Diagnosis Neurodevelopmental Disorders Secretogranin II Case-Control Studies Nanoparticles Medicine Biomarker (medicine) business Signal amplification Biomarkers Biotechnology Neuroscience |
Zdroj: | Scientific Reports, Vol 11, Iss 1, Pp 1-11 (2021) Scientific Reports |
ISSN: | 2045-2322 |
Popis: | The neural circuits of the infant brain are rapidly established near 6 months of age, but neurodevelopmental disorders can be diagnosed only at the age of 2–3 years using existing diagnostic methods. Early diagnosis is very important to alleviate life-long disability in patients through appropriate early intervention, and it is imperative to develop new diagnostic methods for early detection of neurodevelopmental disorders. We examined the serum level of secretogranin II (SCG2) in pediatric patients to evaluate its potential role as a biomarker for neurodevelopmental disorders. A plasmonic immunosensor performing an enzyme-linked immunosorbent assay (ELISA) on a gold nanodot array was developed to detect SCG2 in small volumes of serum. This nanoplasmonic immunosensor combined with tyramide signal amplification was highly sensitive to detect SCG2 in only 5 μL serum samples. The analysis using the nanoplasmonic immunosensor revealed higher serum SCG2 levels in pediatric patients with developmental delay than in the control group. Overexpression or knockdown of SCG2 in hippocampal neurons significantly attenuated dendritic arborization and synaptic formation. These results suggest that dysregulated SCG2 expression impairs neural development. In conclusion, we developed a highly sensitive nanoplasmonic immunosensor to detect serum SCG2, a candidate biomarker for the early diagnosis of neurodevelopmental disorders. |
Databáze: | OpenAIRE |
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