Electrofusion of syngeneic dendritic cells and tumor generates potent therapeutic vaccine
| Autor: | Keiji Shimizu, Jorgen Kjaergaard, Suyu Shu |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Skin Neoplasms
Cell Transplantation medicine.medical_treatment Immunology Antigen presentation Biology Cancer Vaccines Cell Fusion Electrofusion Mice Immune system Antigen Tumor Cells Cultured medicine Animals Cytotoxic T cell Neoplasm Metastasis Mice Inbred BALB C Brain Neoplasms Immunogenicity Immunotherapy Active Dendritic Cells Immunotherapy Mice Inbred C57BL Cancer vaccine |
| Zdroj: | Cellular Immunology. 225:65-74 |
| ISSN: | 0008-8749 |
| DOI: | 10.1016/j.cellimm.2003.09.005 |
| Popis: | Antigen presentation by dendritic cells (DCs) has the potential to elicit therapeutic immune responses against malignant tumors. One strategy utilizing DC-tumor fusion hybrids as cancer vaccine is particularly attractive because of polyclonal presentation of a diverse array of unaltered tumor antigens. We have recently developed a large-scale electrofusion technique for generating DC-tumor heterokaryons and demonstrated their superb immunogenicity. Here, employing the weakly immunogenic MCA205 sarcoma, a single vaccination with electrofusion hybrids eradicated tumors established in the lung, skin, and brain. Immunotherapy required intra-lymphoid vaccine delivery and co-administration of adjuvants such as OX-40R antibody. Tumor eradication was immunologically specific and involved the participation of both CD4 and CD8 T cells. Consistent with DC's functionality of MHC-restriction, the use of syngeneic DCs for fusion was an obligatory requirement. Fusion with allogeneic DCs completely lacked therapeutic effects. These findings provide a strong impetus for treating cancer patients with similarly generated DC-tumor hybrids. |
| Databáze: | OpenAIRE |
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