A new in vitro model for analyzing the biological behavior of well-differentiated squamous cell carcinoma
| Autor: | Akira Inokuchi, Shuji Toda, Shintaro Satoh, Hajime Sugihara |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Biology
Pathology and Forensic Medicine Mice Paracrine signalling Epidermal growth factor Keratin medicine Animals Humans Neoplasm Invasiveness Protein Precursors Receptor Fibroblast Autocrine signalling Involucrin Oligonucleotide Array Sequence Analysis chemistry.chemical_classification Epidermal Growth Factor Hepatocyte Growth Factor Receptors Interleukin-1 Cell Differentiation 3T3 Cells Cell Biology Fibroblasts Phenotype Coculture Techniques Cell biology Ki-67 Antigen medicine.anatomical_structure chemistry Culture Media Conditioned Carcinoma Squamous Cell Mouth Neoplasms Collagen Stromal Cells Gels Cell Division |
| Zdroj: | Pathology - Research and Practice. 201:27-35 |
| ISSN: | 0344-0338 |
| DOI: | 10.1016/j.prp.2004.09.015 |
| Popis: | A suitable model analyzing the behavior of well-differentiated squamous cell carcinoma has not yet been established. We tried to establish such a system using a reconstructed oral mucosa, in which T3M-1 squamous cell carcinoma cells were cultured on 3T3 fibroblast-containing collagen gel. Fibroblasts promoted the stratification and keratinization of T3M-1 cells. During growth, the Ki-67 index of T3M-1 cells with fibroblasts was higher than that of T3M-1 cells alone. Fibroblasts increased the expression of involucrin, a differentiating marker of keratinocytes, in T3M-1 cells. They also promoted the invasion of T3M-1 cells into the gel. When T3M-1 cells alone were cultured in a fibroblast-conditioned (FC) medium, the fibroblast-induced phenomena mentioned above were almost replicated. In addition, epidermal growth factor (EGF) promoted T3M-1 cells growth, but not the invasion. cDNA microarray analysis showed that FC medium increased the expression of EGF receptor and several other mRNAs of T3M-1 cells. The data suggest that T3M-1 cells, under cancer–stromal fibroblast interaction, undergo invasive growth with their well-differentiated squamous phenotype, and that this interaction may be mediated partly by soluble molecules (e.g., EGF) in an autocrine or paracrine pathway. Our system will probably provide a useful model for analyzing the biological behavior of well-differentiated squamous cell carcinoma. |
| Databáze: | OpenAIRE |
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