Influenza A virus reassortants with surface glycoprotein genes of the avian parent viruses: effects of HA and NA gene combinations on virus aggregation
| Autor: | V. P. Kovaleva, I. A. Popova, V. P. Presnova, Irina A. Rudneva, Nikolai V. Kaverin, Gubareva Lv, N. L. Varich, S. S. Yamnikova, V. R. Farashyan |
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| Rok vydání: | 1993 |
| Předmět: |
Genes
Viral viruses Orthomyxoviridae Hemagglutinin (influenza) Hemagglutinins Viral Neuraminidase Chick Embryo medicine.disease_cause Virus chemistry.chemical_compound Viral Proteins Virology Influenza A virus medicine Animals Humans Hemagglutination Viral Infectivity Recombination Genetic Membrane Glycoproteins biology Temperature Whales General Medicine biology.organism_classification Sialic acid Ducks chemistry biology.protein RNA Viral Electrophoresis Polyacrylamide Gel Human Virus Chickens Reassortant Viruses |
| Zdroj: | Archives of virology. 133(3-4) |
| ISSN: | 0304-8608 |
| Popis: | A series of 33 human-avian and human-mammalian influenza virus reassortant clones possessing either HA or both HA and NA genes of the avian or mammalian virus was obtained by crosses of A/USSR/90/77 (H1N1) human virus with 5 avian and 1 mammalian influenza virus strains. All of the reassortants possessing NA genes of the H1N1 human parent virus and HA gene of an avian or mammalian parent virus had high values of infectivity/HA activity ratio. Since this feature could result from a limited virion aggregation, several reassortants were analyzed by velocity sucrose gradient centrifugation. In all cases tested, the reassortants of H3N1, H4N1, H10N1 and H13N1 composition were shown to be aggregated, whereas the preparations of the parent H1N1 virus and the reassortants possessing both HA and NA genes from the avian parents were represented mostly by single virions. The aggregates were formed at 4 degrees C and dissociated at 37 degrees C. The dissociation was blocked by an inhibitor of neuraminidase activity (2-deoxy-2,3-dehydro-N-acetyl-neuraminic acid). The dissociation was reversible since the virions reaggregated at 4 degrees C; however, treatment with bacterial neuraminidase led to an irreversible dissociation of the aggregates. The tendency of the reassortants to aggregate correlates with an increased infectivity/HA ratio. No regular decrease in the neuraminidase activity in the virions of reassortants as compared to the parent H1N1 virus was revealed. The most likely explanation of the observed phenomenon seems to be an inefficient removal of sialic acid residues from the avian virus hemagglutinin by the human virus N1 neuraminidase. |
| Databáze: | OpenAIRE |
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