Antitumor Macrophage Response to Bacillus pumilus Ribonuclease (Binase)
| Autor: | Klaus T. Preissner, Julian Rodriguez-Montesinos, Alexander Nesmelov, A. V. Makeeva, Hector A. Cabrera-Fuentes, Pavel V. Zelenikhin, Olga N. Ilinskaya |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
toll like receptor adaptor molecule 1 animal cell immune response 0302 clinical medicine Bacillus pumilus ribonuclease Bacillus pumilus Toll-like receptor biology toll like receptor unclassified drug cytokine release 030220 oncology & carcinogenesis THP-1 cell line Tumor necrosis factor alpha ribonuclease monocyte chemotactic protein 1 lcsh:RB1-214 Article Subject tumor necrosis factor Immunology transcription factor RelA interleukin 6 macrophage malignant neoplasm interleukin 4 Article 03 medical and health sciences lcsh:Pathology controlled study human Ribonuclease protein expression mouse Interleukin 4 A549 cell polarization nonhuman bacterial enzyme myeloid differentiation factor 88 human cell RNA RAW 264.7 cell line Cell Biology biology.organism_classification Molecular biology 030104 developmental biology 7 INGENIERÍA Y TECNOLOGÍA TRIF biology.protein interleukin 10 |
| Zdroj: | Mediators of Inflammation, Vol 2017 (2017) Mediators of Inflammation |
| ISSN: | 1466-1861 0962-9351 |
| DOI: | 10.1155/2017/4029641 |
| Popis: | Extracellular bacterial ribonucleases such as binase from Bacillus pumilus possess cytotoxic activity against tumor cells with a potential for clinical application. Moreover, they may induce activation of tumor-derived macrophages either into the M1-phenotype with well-documented functions in the regulation of the antitumor immune response or into M2-macrophages that may stimulate tumor growth, metastasis, and angiogenesis. In this study, binase or endogenous RNase1 (but not RNA or short oligonucleotides) stimulated the expression of activated NF-κB p65 subunit in macrophages. Since no changes in MyD88 and TRIF adaptor protein expression were observed, toll-like receptors may not be involved in RNase-related NF-κB pathway activation. In addition, short exposure (0.5 hr) to binase induced the release of cytokines such as IL-6, МСР-1, or TNF-α (but not IL-4 and IL-10), indicative for the polarization into antitumor M1-macrophages. Thus, we revealed increased expression of activated NF-κB p65 subunit in macrophages upon stimulation by binase and RNase1, but not RNA or short oligonucleotides. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|