Cytokine profile and lymphocyte subsets in type 2 diabetes
| Autor: | Silvia Cristina Garcia de Moura-Tonello, Kelen C. R. Malmegrim, Angela M O Leal, Aparecida Maria Catai, A.M. Del Vale, Benedito Galvão Benze, Lucas C. M. Arruda, Cristina de Oliveira Francisco, Sérgio Luiz Brasileiro Lopes |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Adult
Male 0301 basic medicine Physiology T-Lymphocytes medicine.medical_treatment Immunology Biophysics Biology Biochemistry Statistics Nonparametric 03 medical and health sciences Interleukin 21 0302 clinical medicine Immune system Reference Values T-Lymphocyte Subsets Type 2 diabetes mellitus medicine Humans Clinical Investigation Lymphocyte Count IL-2 receptor Lymphocytes General Pharmacology Toxicology and Pharmaceutics lcsh:QH301-705.5 Inflammation lcsh:R5-920 Immunity Cellular General Neuroscience Interleukin FOXP3 Cell Biology General Medicine Middle Aged Flow Cytometry C-Reactive Protein 030104 developmental biology Cytokine lcsh:Biology (General) Diabetes Mellitus Type 2 Case-Control Studies Cytokines Tumor necrosis factor alpha lcsh:Medicine (General) CD8 030215 immunology |
| Zdroj: | Brazilian Journal of Medical and Biological Research v.49 n.4 2016 Brazilian Journal of Medical and Biological Research Associação Brasileira de Divulgação Científica (ABDC) instacron:ABDC Brazilian Journal of Medical and Biological Research, Volume: 49, Issue: 4, Article number: e5062, Published: 18 MAR 2016 Brazilian Journal of Medical and Biological Research, Vol 49, Iss 4 (2016) |
| Popis: | Type 2 diabetes mellitus (T2D) is a metabolic disease with inflammation as an important pathogenic background. However, the pattern of immune cell subsets and the cytokine profile associated with development of T2D are unclear. The objective of this study was to evaluate different components of the immune system in T2D patients' peripheral blood by quantifying the frequency of lymphocyte subsets and intracellular pro- and anti-inflammatory cytokine production by T cells. Clinical data and blood samples were collected from 22 men (51.6±6.3 years old) with T2D and 20 nonsmoking men (49.4±7.6 years old) who were matched for age and sex as control subjects. Glycated hemoglobin, high-sensitivity C-reactive protein concentrations, and the lipid profile were measured by a commercially available automated system. Frequencies of lymphocyte subsets in peripheral blood and intracellular production of interleukin (IL)-4, IL-10, IL-17, tumor necrosis factor-α, and interferon-γ cytokines by CD3+ T cells were assessed by flow cytometry. No differences were observed in the frequency of CD19+ B cells, CD3+CD8+ and CD3+CD4+ T cells, CD16+56+ NK cells, and CD4+CD25+Foxp3+ T regulatory cells in patients with T2D compared with controls. The numbers of IL-10- and IL-17-producing CD3+ T cells were significantly higher in patients with T2D than in controls (P |
| Databáze: | OpenAIRE |
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