Aluminum Exposure from Parenteral Nutrition: Early Bile Canaliculus Changes of the Hepatocyte

Autor: Gordon A. Zello, Amanda R. Hall, Ha Le, Consolato Sergi, Robert F. Bertolo, Janet A. Brunton, Grant G. Miller, Chris J. Arnold
Jazyk: angličtina
Rok vydání: 2018
Předmět:
0301 basic medicine
Parenteral Nutrition
medicine.medical_specialty
Time Factors
Swine
animal diseases
lcsh:TX341-641
Bone canaliculus
liver
Gastroenterology
canaliculus
Article
Bile Acids and Salts
Excretion
Bile canaliculus
03 medical and health sciences
Liver disease
0302 clinical medicine
fluids and secretions
Microscopy
Electron
Transmission
Internal medicine
medicine
Animals
Parenteral Nutrition Solutions
Nutrition and Dietetics
Microvilli
integumentary system
Chemistry
Liver Diseases
Bile Canaliculi
parenteral
toxicity
medicine.disease
Serum samples
030104 developmental biology
Parenteral nutrition
medicine.anatomical_structure
Animals
Newborn
exposure
Hepatocyte
aluminum
Toxicity
Hepatocytes
Swine
Miniature
030211 gastroenterology & hepatology
lcsh:Nutrition. Foods and food supply
Food Science
Zdroj: Nutrients, Vol 10, Iss 6, p 723 (2018)
Nutrients
Volume 10
Issue 6
ISSN: 2072-6643
Popis: Background: Neonates on long-term parenteral nutrition (PN) may develop parenteral nutrition-associated liver disease (PNALD). Aluminum (Al) is a known contaminant of infant PN, and we hypothesize that it substantially contributes to PNALD. In this study, we aim to assess the impact of Al on hepatocytes in a piglet model. Methods: We conducted a randomized control trial using a Yucatan piglet PN model. Piglets, aged 3&ndash
6 days, were placed into two groups. The high Al group (n = 8) received PN with 63 µ
g/kg/day of Al, while the low Al group (n = 7) received PN with 24 µ
g/kg/day of Al. Serum samples for total bile acids (TBA) were collected over two weeks, and liver tissue was obtained at the end of the experiment. Bile canaliculus morphometry were studied by transmission electron microscopy (TEM) and ImageJ software analysis. Results: The canalicular space was smaller and the microvilli were shorter in the high Al group than in the low Al group. There was no difference in the TBA between the groups. Conclusions: Al causes structural changes in the hepatocytes despite unaltered serum bile acids. High Al in PN is associated with short microvilli, which could decrease the functional excretion area of the hepatocytes and impair bile flow.
Databáze: OpenAIRE
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