Production of acetoin from renewable resources under heterotrophic and mixotrophic conditions
| Autor: | Carina Windhorst, Johannes Gescher, Yohann Couté, Julia Novion Ducassou, Daniel Härrer, Nicola Böhner |
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| Přispěvatelé: | Etude de la dynamique des protéomes (EDyP), BioSanté (UMR BioSanté), Université Grenoble Alpes (UGA)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Grenoble Alpes (UGA)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes (UGA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes (UGA), Laboratoire Biosciences et bioingénierie pour la santé (BGE) |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
Proteomics Environmental Engineering Cupriavidus necator [SDV]Life Sciences [q-bio] Bioengineering Butyrate 010501 environmental sciences 01 natural sciences 7. Clean energy Polyhydroxybutyrate chemistry.chemical_compound 010608 biotechnology Food science Autotroph Waste Management and Disposal ComputingMilieux_MISCELLANEOUS 0105 earth and related environmental sciences chemistry.chemical_classification Autotrophic Processes biology Renewable Energy Sustainability and the Environment Acetoin Heterotrophic Processes General Medicine Dark fermentation biology.organism_classification chemistry Propionate Mixotroph |
| Zdroj: | Bioresource Technology Bioresource Technology, Elsevier, 2021, 329, pp.124866. ⟨10.1016/j.biortech.2021.124866⟩ Bioresource Technology, 2021, 329, pp.124866. ⟨10.1016/j.biortech.2021.124866⟩ Bioresource Technology, 2021, 329 (17), pp.124866. ⟨10.1111/pce.14013⟩ |
| ISSN: | 0960-8524 |
| DOI: | 10.1016/j.biortech.2021.124866⟩ |
| Popis: | This study aimed to reveal whether Cupriavidus necator H16 is suited for the production of acetoin based on the carboxylic acids acetate, butyrate and propionate under heterotrophic and mixotrophic conditions. The chosen production strain, lacking the polyhydroxybutyrate synthases phaC1 and phaC2, was revealed to be beneficiary for autotrophic acetoin production. Proteomic analysis of the strain determined that the deletions do indeed have a significant impact on pyruvate formation and its subsequent direction towards the introduced acetoin-synthesis pathway. Moreover, the strain was tested for its ability to use typical dark fermentation products under hetero- and mixotrophic conditions. Growth with butyrate and acetate led to low efficiencies, while 46.54% ±0.78 of the added propionate was converted into acetoin. Interestingly, mixotrophic conditions led to simultaneous consumption of acetate and butyrate with the gaseous substrates and lowered efficiency. In contrast, mixotrophic propionate consumption led to diauxic behavior and high carbon efficiency of 71.2% ±0.64. |
| Databáze: | OpenAIRE |
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