In vivo wound-healing efficacy and antioxidant activity of Achyranthes aspera in experimental burns
| Autor: | D. K. Sarma, Rumi S. Borah, Chandana Choudhury Barua, Archana Talukdar, D. C. Pathak, Asheesh Gupta, Shameem Ara Begum |
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| Rok vydání: | 2012 |
| Předmět: |
Male
Antioxidant medicine.medical_treatment Administration Topical Pharmaceutical Science Antioxidants Hydroxyproline chemistry.chemical_compound Mice Drug Discovery medicine Animals Achyranthes Pharmacology Wound Healing integumentary system biology Traditional medicine Achyranthes aspera Plant Extracts food and beverages Granulation tissue General Medicine Amaranthaceae biology.organism_classification Ascorbic acid Rats Plant Leaves Disease Models Animal medicine.anatomical_structure Treatment Outcome Complementary and alternative medicine chemistry Molecular Medicine Female Wound healing Burns |
| Zdroj: | Pharmaceutical biology. 50(7) |
| ISSN: | 1744-5116 |
| Popis: | The floral richness of the North-East Indian region cannot be neglected in context to its medicinal importance. Achyranthes aspera Linn. (Amaranthaceae; Prickly Chaff flower) is an indigenous plant species of this region. Although the local traditional healers have ethnomedical knowledge on the use of this plant, there is no scientific study on wound-healing activity of this plant.The healing efficacy of methanol leaf extract of A. aspera (MEAA) in granulation tissue of burn wound and its antioxidant activity are investigated.Methanol extract of leaves of A. aspera was used for compounding 5% (w/w) ointment, which was applied topically twice daily in experimental burn wound in rats. Healing potential was assessed by rate of wound contraction, antioxidant and biochemical assay which was supported by gelatin zymography and histopathology.In the present study, 5% ointment of A. aspera showed significant (p0.05) wound healing, which was evident by wound contraction, elevation of various antioxidant enzymes viz. SOD, catalase, vitamin C and prohealing and biochemical parameters like hydroxyproline and protein content than the control animals. Up-regulated expression of matrix metalloproteinases (MMP-2 and 9) was also observed by gelatin zymography. Histopathological examination of the granulation tissues in the A. aspera-treated animals showed collagen deposition, fibroblast proliferation and formation of epidermis.The methanol leaf extract of A. aspera showed excellent wound-healing activities which has great potential for development of plant-based product. |
| Databáze: | OpenAIRE |
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