Ovarian toxicity by fusariotoxins in pigs: Does it imply in oxidative stress?
| Autor: | Miriam S. N. Hohmann, Juliana Rubira Gerez, Thaynara Camacho, Victor Hugo Brunaldi Marutani, Ricardo Luís Nascimento de Matos, Ana Paula Frederico Rodrigues Loureiro Bracarense, Waldiceu Aparecido Verri Junior |
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| Rok vydání: | 2021 |
| Předmět: |
Antioxidant
Swine medicine.medical_treatment Biology medicine.disease_cause Fumonisins Lipid peroxidation Andrology 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Food Animals medicine Animals Small Animals Granulosa cell proliferation Fumonisin B1 030219 obstetrics & reproductive medicine Equine Cell growth Ovary 0402 animal and dairy science food and beverages 04 agricultural and veterinary sciences Mycotoxins 040201 dairy & animal science Oxidative Stress T-2 Toxin chemistry Apoptosis Female Animal Science and Zoology Trichothecenes Reproductive toxicity Oxidative stress |
| Zdroj: | Theriogenology. 165:84-91 |
| ISSN: | 0093-691X |
| Popis: | Mycotoxins are natural contaminants of food and feed occurring worldwide. Deoxynivalenol (DON) and fumonisin B1 (FB1) are the most frequent fusariotoxins and induce immune and intestinal toxicity in humans and animals. Recently, an association between mycotoxins exposure and impaired fertility has been suggested. However, the effects of these mycotoxins on the reproductive system are not well established. This study aimed to evaluate the effects of FB1 and DON, in combination or alone, on the ovarian morphology and oxidative responses using porcine explants. Seventy-two explants were obtained from six pigs and submitted to the following treatments: control (MEM medium), DON (10 μM), FB1 (100 μM FB1), and DON + FB1 (10 μM + 100 μM). Histological and immunohistochemical assays were performed to evaluate ovarian changes, cell proliferation, and apoptosis. Oxidative stress response was evaluated through lipid peroxidation and antioxidant capacity response assays. The exposure to mycotoxins induced significant histological changes in the ovaries, which were characterized by a decrease in viable follicles and increase in degenerated follicles. A significant decrease in granulosa cell proliferation was observed in explants exposed to all mycotoxins. In addition the multi-contaminated treatment was responsible for an increase in the cell apoptosis index of growing follicles. On the other hand, the FB1 and multi-contaminated treatments induced a significant decrease in lipid peroxidation accompanied by an increase in antioxidant responses. Altogether, our results indicate a reproductive toxicity induced by fusariotoxins. Moreover, mycotoxins, alone or in combination, modulate oxidative stress response, interfering with the production of free radicals and affecting the reproductive capacity of pigs. |
| Databáze: | OpenAIRE |
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