The LXCXE Retinoblastoma Protein-Binding Motif of FOG-2 Regulates Adipogenesis
| Autor: | Raphael G. P. Denis, Leila Maouche-Chretien, Marine Granger-Locatelli, Cécile Badoual, Suthat Fucharoen, Stany Chrétien, Philippe Leboulch, Serge Luquet, Olivier Goupille, Tipparat Penglong, Zahra Kadri |
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| Přispěvatelé: | Institut de Biologie François JACOB (JACOB), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Mahidol University [Bangkok], Unité de Biologie Fonctionnelle et Adaptative (BFA (UMR_8251 / U1133)), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), Hôpital Européen Georges Pompidou [APHP] (HEGP), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO), Université Paris Descartes - Paris 5 (UPD5), Brigham and Women's Hospital [Boston], Harvard Medical School [Boston] (HMS), Université Paris Diderot - Paris 7 (UPD7)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), ORANGE, Colette |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
EXPRESSION DIET-INDUCED OBESITY Cell division genetic structures Adipose Tissue White [SDV]Life Sciences [q-bio] Amino Acid Motifs LACTATE General Biochemistry Genetics and Molecular Biology Cell Line 03 medical and health sciences Mice Adipose Tissue Brown GATA TRANSCRIPTION FACTORS BROWN FAT medicine Animals Obesity E2F Gene lcsh:QH301-705.5 Tissue homeostasis Cell Proliferation Adipogenesis biology Retinoblastoma Retinoblastoma protein ENERGY-EXPENDITURE medicine.disease Cell biology [SDV] Life Sciences [q-bio] DNA-Binding Proteins ZINC-FINGER PROTEINS 030104 developmental biology ADIPOSE-TISSUE DIFFERENTIATION lcsh:Biology (General) Mutation biology.protein GATA transcription factor WHITE Transcription Factors |
| Zdroj: | Cell Reports Cell Reports, 2017, 21 (12), pp.3524-3535. ⟨10.1016/j.celrep.2017.11.098⟩ Cell Reports, Elsevier Inc, 2017, 21 (12), pp.3524-3535. ⟨10.1016/j.celrep.2017.11.098⟩ Cell Reports, Vol 21, Iss 12, Pp 3524-3535 (2017) |
| ISSN: | 2211-1247 |
| Popis: | Summary: GATA transcription factors and their FOG cofactors play a key role in tissue-specific development and differentiation, from worms to humans. Mammals have six GATA and two FOG factors. We recently demonstrated that interactions between retinoblastoma protein (pRb) and GATA-1 are crucial for erythroid proliferation and differentiation. We show here that the LXCXE pRb-binding site of FOG-2 is involved in adipogenesis. Unlike GATA-1, which inhibits cell division, FOG-2 promotes proliferation. Mice with a knockin of a Fog2 gene bearing a mutated LXCXE pRb-binding site are resistant to obesity and display higher rates of white-to-brown fat conversion. Thus, each component of the GATA/FOG complex (GATA-1 and FOG-2) is involved in pRb/E2F regulation, but these molecules have markedly different roles in the control of tissue homeostasis. : Goupille et al. find that a mutation of the FOG-2 LXCXE pRb-binding site decreases cell proliferation and affects adipogenesis in vitro and in vivo. Fog2Rb−/Rb− mutant mice are resistant to obesity, and they present abnormal WAT/BAT conversion and lactate production. Oxamate treatment results in phenotype reversion in these mice. Keywords: FOG-2, GATA, retinoblastoma, WAT, BAT, adipogenesis, obesity |
| Databáze: | OpenAIRE |
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