Development of a standardized susceptibility test for campylobacter with quality-control ranges for ciprofloxacin, doxycycline, erythromycin, gentamicin, and meropenem
| Autor: | M. M. Johnson, Stanley Mirrett, Sonya M. Bodeis, D. Shortridge, I. A. Critchley, Patrick F. McDermott, Robert D. Walker, Sharon Schooley, C. Thornsberry, J. Beyer, Robert Rennie, Gary E. Stein, R. Flamm, Steven D. Brown, R. A. Hanson, Ronald N. Jones, C. Brosnikoff, S. Graff, Laura J. V. Piddock, Paula J. Fedorka-Cray, Frank Møller Aarestrup, J. Aldrobi, M. Wallace, M. Traczewski, L. Turnbull, Vito Ricci, B. Reller |
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| Rok vydání: | 2004 |
| Předmět: |
Microbiology (medical)
Quality Control Immunology Erythromycin Microbial Sensitivity Tests Biology medicine.disease_cause Microbiology Meropenem Campylobacter jejuni Agar dilution Minimum inhibitory concentration Ciprofloxacin medicine Humans Pharmacology Campylobacter bacterial infections and mycoses biology.organism_classification Doxycycline Gentamicin Thienamycins Gentamicins medicine.drug |
| Zdroj: | Microbial drug resistance (Larchmont, N.Y.). 10(2) |
| ISSN: | 1076-6294 |
| Popis: | A standardized agar dilution susceptibility testing method was developed for Campylobacter that consisted of testing on Mueller-Hinton medium supplemented with 5% defibrinated sheep blood in an atmosphere of 10% CO2, 5% O2, and 85% N2. Campylobacter jejuni ATCC 33560 was identified as a quality-control (QC) strain. Minimal inhibitory concentration (MIC) QC ranges were determined for two incubation time/temperature combinations: 36 degrees C for 48 hr and 42 degrees C for 24 hr. Quality-control ranges were determined for ciprofloxacin, doxycycline, erythromycin, gentamicin, and meropenem. For all antimicrobial agents tested at both temperatures, 95-100% of the QC MIC results fell within recommended QC ranges. Twenty-one Campylobacter clinical isolates, encompassing five species of Campylobacter (C. jejuni, C. coli, C. jejuni, subsp. doylei, C. fetus, and C. lari) were tested in conjunction with the C. jejuni QC strain. While C. jejuni and C. coli could be reliably tested under both test conditions, growth of C. jejuni subsp. doylei, C. fetus, and C. lari isolates was inconsistent when incubated at 42 degrees C. Therefore, it is recommended that these species only be tested at 36 degrees C. |
| Databáze: | OpenAIRE |
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