An electrophoretic karyotype of the cultivated mushroom--Agaricus bisporus
| Autor: | David Wood, Keith Gull, Stephen Lodder |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1993 |
| Předmět: |
Agaricus
Saccharomyces cerevisiae Biology DNA Ribosomal chemistry.chemical_compound Cellulase Gene mapping Candida albicans Schizosaccharomyces Genetics DNA Fungal Southern blot Electrophoresis Agar Gel Gene Rearrangement Heterokaryon Base Composition Laccase Chromosome Mapping Chromosome Karyotype General Medicine Gene rearrangement Molecular biology Molecular Weight chemistry Karyotyping Chromosomes Fungal Oxidoreductases DNA Agaricus bisporus |
| Zdroj: | Current genetics. 24(6) |
| ISSN: | 1432-0983 0172-8083 |
| Popis: | Thirteen chromosomal-sized DNA bands of the cultivated mushroom Agaricus bisporus have been resolved using the method of clamped homogeneous electric field (CHEF) electrophoresis. Using chromosome size standards from Schizosaccharomyces pombe, Saccharomyces cerevisiae and Candida albicans, the estimated size of the chromosomal DNAs ranged from 3.5 to 1.2 megabase pairs (Mb). By Southern hybridization with homologous gene probes, the chromosomal location of cellulase and laccase genes have been mapped. In addition, rDNA has been assigned to chromosomal bands using a heterologous gene probe. Genomic rearrangement is suggested in the commercial heterokaryon, as indicated by the presence of non-comigrating homologous chromosomes, identified by a number of probes for particular DNA sequences. |
| Databáze: | OpenAIRE |
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