Cytokines Levels and Salivary Microbiome Play A Potential Role in Oral Lichen Planus Diagnosis
| Autor: | Maria Fernanda Marques Silva de Carvalho, Luciana Cavalheiro Marti, Debora Heller, Denise da Cunha Pasqualin, Fernanda Agostini Rocha, Danielle Viana Ribeiro Ramos, Sabrina Do Nascimento, Denise Cavalieri, Leandro Aurélio Liporoni Martins, Talita Gomes Baêta Lourenço |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Male Saliva medicine.medical_treatment Mucocutaneous zone lcsh:Medicine Gene Expression Article 03 medical and health sciences Interferon-gamma 0302 clinical medicine stomatognathic system Oral and maxillofacial pathology medicine Humans Microbiome Oral mucosa lcsh:Science Aged Aged 80 and over Multidisciplinary business.industry Interleukins Microbiota lcsh:R Interleukin-17 Mouth Mucosa Middle Aged medicine.disease Interleukin-33 Peptide Fragments stomatognathic diseases 030104 developmental biology medicine.anatomical_structure Cytokine 030220 oncology & carcinogenesis Oral microbiology Immunology Mucosal immunology Cytokines Oral lichen planus lcsh:Q Female business Biomarkers Lichen Planus Oral |
| Zdroj: | Scientific Reports Scientific Reports, Vol 9, Iss 1, Pp 1-10 (2019) |
| ISSN: | 2045-2322 |
| Popis: | Oral lichen planus (OLP) is a chronic Th1-mediated inflammatory mucocutaneous disease of the skin and oral mucosa that can have various clinical presentations. Lesions are usually bilateral and often painful. While cutaneous Lichen Planus (LP) lesions are self-limiting, the oral lesions are chronic and rarely remissive. The diagnosis of oral lichen planus (OLP) is often challenging, and confirmation by histopathological criterion is generally advised. The aim of our study was to identify the cytokines present in OLP-suggestive lesions and in non-specific inflammatory lesions (NSIL) used as controls. Moreover, assess cytokines protein levels and oral microbiota composition in whole saliva samples. Histopathological analysis, immunohistochemistry and gene expression were used as techniques to analyze the oral mucosal tissue samples. ELISA was conducted to analyze salivary cytokine levels and 16S rRNA sequencing was used to determine the salivary microbiome. As a result we observed larger number of infiltrated lymphocytes (p = 0.025), as well, more T CD4 lymphocytes in the epithelial tissue (p = 0.006) in OLP samples compared to NSIL. In addition, the OLP samples displayed more apoptotic cells compared to NSIL (p = 0.047). Regarding the cytokine analysis, IFN-γ and IL-33 were more expressed in OLP lesions than in NSIL samples (p |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|