Isolation and Identification ofEnterobacter sakazakiiin Infant Milk Formulas
| Autor: | Elsa Ramírez-Cerda, Edith Torres-Chavolla, Rosalba Gutiérrez-Rojo |
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| Rok vydání: | 2007 |
| Předmět: |
Time Factors
food.ingredient Food industry Colony Count Microbial Food Contamination Applied Microbiology and Biotechnology Microbiology food Cronobacter sakazakii medicine White light Humans Agar Food science Mexico Alternative methods Bacteriological Techniques biology Pigmentation business.industry Infant Newborn Infant Enterobacter medicine.disease biology.organism_classification Isolation (microbiology) Infant Formula Culture Media Infant formula Necrotizing enterocolitis Food Microbiology Infant Food Animal Science and Zoology Powders business Food Science |
| Zdroj: | Foodborne Pathogens and Disease. 4:164-168 |
| ISSN: | 1556-7125 1535-3141 |
| DOI: | 10.1089/fpd.2006.0071 |
| Popis: | Enterobacter sakazakii is a pathogen of increasing medical concern, due to it being implicated in cases of meningitis, sepis, and necrotizing enterocolitis associated with the consumption of contaminated infant milk formula. At present, the method adopted by the Mexican food industry for the isolation and identification of E. sakazakii is based on the methodology of the United States Food and Drug Administration (FDA). However, this procedure is laborious and requires 7 days to obtain a confirmative result. The objective of this study was to determine the presence of E. sakazakii in two types of powdered infant milk formula, using an alternative method that requires less time and a smaller sample size than the FDA protocol. We adapted Leuschner's procedure by eliminating violet red bile glucose agar (VRBG) plates and instead adopting white light incubation to stimulate yellow pigment development. This allowed for isolation of E. sakazakii from powdered infant milk formula using a smaller sample and requiring only 5 days for analysis. Results showed that 92% of formula 1 and 32% of formula 2 was positive for E. sakazakii. The high contamination level of E. sakazakii suggests the need for monitoring hygienic conditions in the manufacturing plant and to assess the prevalence of E. sakazakii in powdered infant milk formulas sold in México. |
| Databáze: | OpenAIRE |
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