THE EFFECTS OF ETANERCEPT AND CABERGOLINE ON ENDOMETRIOTIC IMPLANTS, UTERUS AND OVARIES IN RAT ENDOMETRIOSIS MODEL
| Autor: | Fatma Ceyla Eraldemir, Fisun Vural, Cihan Deniz Keleş, Sema Kurnaz, Birol Vural, Serdar Filiz, Gulcin Gacar |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Vascular Endothelial Growth Factor A
0301 basic medicine medicine.medical_specialty Cabergoline Immunology Drug Evaluation Preclinical Endometriosis Uterus Ovary Etanercept Endometrium 03 medical and health sciences 0302 clinical medicine Internal medicine Animals Humans Immunology and Allergy Medicine 030219 obstetrics & reproductive medicine Tumor Necrosis Factor-alpha business.industry Obstetrics and Gynecology medicine.disease Pathophysiology Rats Disease Models Animal 030104 developmental biology medicine.anatomical_structure Endocrinology Reproductive Medicine Immunohistochemistry Drug Therapy Combination Female Tumor necrosis factor alpha business medicine.drug |
| Zdroj: | Journal of Reproductive Immunology. 146:103340 |
| ISSN: | 0165-0378 |
| Popis: | The pathophysiology of endometriosis is still unknown and treatment options remain controversial. Searches focus on angiogenesis, stem cells, immunologic and inflammatory factors. This study investigated the effects of etanercept and cabergoline on ovaries, ectopic, and eutopic endometrium in an endometriosis rat model. This randomized, placebo-controlled, blinded study included 50 rats, Co(control), Sh(Sham), Cb(cabergoline), E(etanercept), and E + Cb(etanercept + cabergoline) groups. After surgical induction of endometriosis, 2nd operation was performed for endometriotic volume and AMH level. After 15 days of treatment: AMH level, flow cytometry, implant volume, histologic scores, immunohistochemical staining of ectopic, eutopic endometrium, and ovary were evaluated at 3rd operation. All groups had significantly reduced volume, TNF-α, VEGF, and CD 146/PDGF-Rβ staining of endometriotic implants comparing to the Sh group (p 0.05). E and E + Cb groups significantly decreased TNF-α staining in the ovary comparing to Sh, Co, and Cb groups (p < 0.05). All treatment groups had significantly higher AFC compared to the Sh group. CD25+ Cells' median percentage was significantly increased in the E + Cb group compared to Co, Sh, Cb, and E group. E + Cb group had a significantly higher CD5+ Cells' level than the Co group (p = 0.035). In conclusion; Etanercept and/or Cabergoline decreased volume, TNF-α, VEGF, and CD 146/PDGF-Rβ staining of the ectopic endometrial implant. E and E + Cb treatment decreased TNF-α levels in the ovary. E + Cb also increased peripheral blood CD25+ & CD5+ Cell's. |
| Databáze: | OpenAIRE |
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